锌对脊髓缺血再灌注损伤大鼠ATP酶、MAO活性和T-AOC含量影响的研究

Effect of zinc on the ATP enzyme、MAO activity and T-AOC contentof rats with spinal cord ischemia-reperfusion injury

目的方法研究外源性补锌对脊髓缺血再灌注损伤大鼠ATP酶、MAO活性和T-AOC含量的影响,从而探讨锌对脊髓缺血再灌注损伤的保护作用的机制。方法 60只Wistar大鼠随机分为5组,每组12只,雌雄各半,分别为假手术组、模型组、硫酸锌预处理高剂量组(100mg/kg)、中剂量组(50mg/kg)和低剂量组(25mg/kg)。各剂量组每日灌胃给予硫酸锌1次,连续7天。假手术组和模型组给予相同剂量生理盐水,7天后制备脊髓缺血再灌注损伤动物模型,损伤后24h采用Basso Beattie Bresnahan(BBB)运动功能评分法对脊髓缺血再灌注损伤模型进行后肢神经功能评价;测定脊髓组织中ATP酶、MAO活性和T-AOC含量。结果与模型组比较,各给锌组大鼠神经功能评分明显高于模型组,差异具有显著性(P<0.05)。各硫酸锌处理组Na+-K+-ATP酶活性明显低于假手术组(P<0.05),但明显高于模型组(P<0.05)。模型组和各硫酸锌处理组脊髓组织Ca2+-Mg2+-ATP酶活性显著低于假手术组(P<0.05)。模型组和各硫酸锌处理组大鼠脊髓组织内的MAO活性均显著高于假手术组(P<0.05),且100mg/kg/d硫酸锌处理组MAO活性显著低于模型组(P<0.05)。模型组和各硫酸锌处理组大鼠脊髓组织内的T-AOC含量显著低于假手术组(P<0.05)。模型组大鼠脊髓组织中T-AOC含量最低,随着硫酸锌剂量的增加,T-AOC含量呈上升趋势。结论锌可能通过提高ATP酶的活性、抑制MAO活性和升高T-AOC含量,从而对脊髓缺血再灌注损伤起到一定的保护作用。

Objective To study the effect of the exogenous zinc supplement on the ATP enzyme、MAO activity and T-AOC content of rats with spinal cord ischemia-reperfusion inj ury in order to explore the mechanism of protective effect of zinc on the spinal cord ischemia-reperfusion injury.Methods 60 Wistar rats were randomly divided into five groups：sham-operation group,model group,and zinc sulfate pre-treatment groups including high-dose zinc group （100 mg/kg）,middle-dose zinc group （50 mg/kg）and low-dose zinc group（25 mg/kg）,12 rats in each group,half male and half female.In all the zinc pre-treatment groups,rats were administered zinc sulfate one time by gavage in seven days succession.The rats in sham-operation group and model group were administered the same volume normal saline solu-tion as zinc pre-treatment group.Spinal cord ischemia-reperfusion inj ury model was established after zinc pretreatment for 7days.24 h after injury,Motor function assessment of rats were performed by Basso Beattie Bresnahan （BBB）Mo-tor Function Assessment;ATP enzyme,MAO activity and T-AOC content in spinal cord were detected.Results BBB motor function assessment of zinc group were significantly higher than that of model group （P〈0.05）.Na^＋-K^＋-ATP enzyme activity in zinc pre-treatment groups was significantly lower than that in sham-operation group（P〈0.05）,but significantly higher than that in model group（P〈0.05）.Ca^2＋-Mg^2＋-ATP enzyme activity in rats in model group and zinc pre-treatment groups was significantly lower than that in sham-operation group（P〈0.05）.MAO activity in rats in model group and zinc pre-treatment groups significantly higher than that in sham-operation group（P〈0.05）.And MAO activity in rats in high-dose zinc group （100 mg/kg）was significantly lower than that in model group （P〈0.05）. T-AOC activity in rats in model group and zinc pre-treatment groups was significantly lower than that in sham-opera-tion group （P〈0.05）,T-AOC content in model group was the lowest and increased with the increasing of zinc dose. Conclusion zinc has a protective effect on spinal cord ischemia-reperfusion inj ury by possibly.