肾移植术后T淋巴细胞亚群和NK细胞百分比参考范围的研究

Study on reference values of T-lymphocyte subsets and NK cell percentage after renal transplant operation

目的初步建立肾移植患者外周血T淋巴细胞亚群和NK细胞的百分比参考范围。方法 67例健康对照组、169例肾移植患者于肾移植手术后1周清晨采集外周血,用流式细胞仪检测T淋巴细胞亚群和NK细胞百分比。同时按年龄段划分为3组进行统计：≤30岁（青年组）、31-49岁（中年组）、≥50岁（老年组）。结果 （1）建立了肾移植术后T淋巴细胞亚群和NK细胞百分比的参考范围：CD3^＋56.80%-64.54%;CD4^＋30.82%-36.43%;CD8^＋23.45%-27.32%;CD3^＋CD4^＋/CD3^＋CD8^＋1.35-1.68;NK 6.04%-8.35%。（2）肾移植术后不同性别间的T淋巴细胞亚群和NK细胞差异无统计学意义（P〉0.05）。（3）肾移植术后不同年龄间的T淋巴细胞亚群和NK细胞差异有统计学意义（P〈0.05）：与老年组比较,青年组和中年组肾移植术后CD8^＋细胞百分比明显升高,差异有统计学意义（P〈0.01）,CD3^＋CD4^＋/CD3^＋CD8^＋明显降低,差异有统计学意义（P〈0.01）。结论初步建立了肾移植术后的T淋巴细胞亚群和NK细胞的参考范围,同时发现肾移植术后随年龄增长CD8＋细胞百分比降低,而CD3^＋CD4^＋/CD3^＋CD8^＋升高。

Objective To preliminarily establish the reference values of peripheral blood T‐lymphocyte subsets and NK cell percentage in renal transplant patients .Methods Peripheral blood was collected from 67 cases of control and 169 cases of renal transplant in the early morning at postoperative 1 week .T‐lymphocyte subsets and NK cell percentage were detected by the flow cytometry .169 patients with renal transplant were divided into three groups ac‐cording to age section for conducting statistics：≤30 years old（youth group） ,31-49 years old（middle age group） and≥50 years old（older age group） .Results （1）The reference values of T‐lymphocyte subsets and NK cell percentage were established：CD3^＋ ：56 .80% -64 .54% ;CD4^＋ ：30 .82% -36 .43% ;CD8^＋ ：23 .45% -27 .32% ;CD3^＋ CD4^＋ /CD3^＋CD8^＋ ：1 .35% -1 .68% ;NK ：6 .04% -8 .35% .（2） T‐lymphocyte subsets and NK cell percentage showed no statistical differences between genders（P〉0 .05） .（3） T‐lymphocyte subsets and NK cell percentage showed statisti‐cal differences among different ages（P〈0 .05） .Compared with the older age group ,the level of CD8＋ in the youth group and the middle age group was significantly increased ,while CD3^＋ CD4^＋ /CD3^＋ CD8^＋ was significantly de‐creased（P〈0 .01） .Conclusion The reference values of T lymphocyte subsets and NK cell percentage in renal trans‐plant patients are preliminarily established .Meanwhile it is found that the level of CD8＋ is decreased with the age in‐crease after renal transplant ,while CD3^＋CD4^＋ /CD3^＋CD8^＋ is increased .