实用McAb-A-E直接法检验T淋巴细胞亚群实验操作步骤量化控制研究

Study on testing T cell subsets by pratical McAb-A-E with quantized control in operational process

目的对单克隆抗体SPA红细胞花环法(McAb-A-E)直接法试验操作步骤进行量化控制,从而对原方法进行改良。方法采用改良法与原方法对23例患者外周血中的淋巴细胞表面抗原CD3+、CD4+、CD8+进行检测,并用配对t检验对两种方法的结果进行对比。改良法的量化控制主要是用一定容积的移液器混匀替代"轻轻摇匀",以定量细胞悬液制作一定面积涂片,规范涂片细胞计数区域。结果改良法与原方法检测的CD3+、CD4+、CD8+及CD4+与CD8+的比值(CD4+/CD8+)结果比较差异均无统计学意义(P>0.05)。结论改良后的实用McAb-A-E直接法对细胞悬液混匀方法、细胞涂片制作和细胞计数区域进行了量化控制,可将试验操作误差降到最低。

Objective To quantize key process of direct McAb-A-E including gently shake, cell smear making and ceil count, which would make some steps easier and result exactly. Methods A total of 23 samples were detected lymphocyte antigen levels of CD3＋ ,CD4＋ ,CD8＋ by the original method and quantized method. In the quantized method,gently shake was replaced by pipettor, cell smear was made by quantized cell suspension,arid cell count region was operated with standard. Results There were no signifi- cant differences on the level of CD3 ＋ ,CD4 ＋ ,CD8 ＋ , and the ratio of CD4＋ and CD8＋ between quantized method and original method （P〉0.05）. Conclusion Quantizing the key process of McAb-A-E including gently shake, cell smear making and cell count could reduce error in test procedures.