流式细胞术检测二期梅毒患者外周血淋巴细胞亚群

Flow cytometric detection of peripheral blood lymphocyte subsets in patients with secondary syphilis

目的检测二期梅毒外周血淋巴细胞亚群,了解机体在二期梅毒的免疫状况。方法选择本院门诊就诊、体检或术前检查的35例志愿者,按照其检测结果将其分为健康对照组与二期梅毒组。应用流式细胞仪检测两组患者外周血CD3+、CD4+、CD8+和CD19+淋巴细胞亚群。结果二期梅毒组CD3+和CD4+均高于健康对照组,差异有统计学意义(P=0.008 5,0.038 4),CD8+、CD4+/CD8+高于健康对照组,但差异无统计学意义(P=0.210 8,0.395 2);二期梅毒组CD19+B淋巴细胞低于健康对照组,差异有统计学意义(P=0.006 0)。结论增强二期梅毒患者细胞免疫力有利于消除梅毒螺旋体。

Objective To detect lymphocyte subpopulations in patients with secondary syphilis and to know their immunity con- dition. Methods Flow cytometry was used to detect CD3＋ ,CD4＋ ,CD8＋ and CD19＋ lymphocyte in peripheral blood from 35 volun- teers,including 15 patients with secondary syphilis and 20 normal human. Results There were significant differences in CD3＋ and CD4＋ （P=0. 008 5,0. 038 4） ,CD3＋ and CD4＋ in secondary syphilis group were higher than that in the normal group. There were not significant differences in CD8＋ and CD4＋/CD8＋ （P=0. 210 8,0. 395 2）. There were significant differences in CD19＋ （P=0. 0060） ,CD19＋ in the normal group were higher than that in the secondary syphilis group. Conclusion Enhancing the immunity Of pa- tients with secondary syphilis is benefit to eliminating treponema pallidum.