Sysmex UF-500i检测尿红细胞影响因素的探讨

Effect factors of Sysmex UF-500i in detecting urinary red cell

目的对Sysmex UF-500i全自动型尿沉渣分析仪在红细胞(RBC)检测中的影响因素进行探讨。方法采用Sysmex UF-500i和显微镜两种方法分别对该院门诊患者870份新鲜尿液标本进行RBC检查和计数,并对结果进行比较。结果 Sysmex UF-500i检测尿RBC阳性率为30.0%,显微镜镜检阳性率为24.0%,两种检验方法的阳性率比较差异有统计学意义(P<0.01)。Sysmex UF-500i检测尿RBC会出现假性结果,结晶、真菌孢子、细菌群是导致假阳性的主要因素,而破碎RBC和影形RBC则是导致假阴性结果的主要影响因素。结论临床上采用Sysmex UF-500i全自动尿沉渣分析仪检测尿RBC时,需结合显微镜镜检以避免出现假阳性和假阴性的结果。

Objective To investigate influence factors of Sysmex UF-500i automated urinary sediment analyzer in detecting red blood cells. Methods A total of 870 clinical urine samples were detected by Syemex UF-500i automated urinary sediment analyzer and microscopy,the results of two methods were compared. Results The positive rate of the red blood cells in urine samples detec- ted by the Syemex UF-500i and microscope were 30.0% and 24.0; respectively,there was a significant difference between them. Crystallizations,fungal spores,bacteria group were the main factors causing the false positive result of Syemex UF-500i. In addi- tion, crushing red blood cells and shadow red blood cells were the main factors causing the false negative result of Syemex UF-500i. Conclusion Sysmex UF-500i could check the urine red blood cells quickly and accurately,but should be combine microscopy to a- void false positive and false negative result.