电针对局灶性脑缺血大鼠10号染色体同源丢失性磷酸酶张力蛋白和神经生长相关蛋白-43表达的影响

Effects of Electroacupuncture on Expressions of Phosphatase and Tensin homology Deleted on Chromosome Ten and Growth Associated Protein 43 in Rats with Barrel Cortex Ischemia

目的探讨电针对胡须体觉皮层局灶性脑缺血大鼠10号染色体同源丢失性磷酸酶张力蛋白(PTEN)和神经生长相关蛋白43(GAP-43)表达的影响。方法 24只成年健康雄性Sprague-Dawley大鼠分为假手术组(n=8)、模型组(n=8)及电针组(n=8)。于造模成功后第3天,电针组予电针百会(DU20)和风府(DU16)30 min,每天1次。造模前1 d,给药后3 d、7 d和14 d,应用角落测试进行行为学检测;给药后14 d采用Western blotting检测梗死周边区PTEN、GAP-43蛋白的表达。结果电针组向右转的次数较模型组显著下降(P<0.001)。模型组GAP-43表达较假手术组升高(P<0.05),电针组较模型组升高(P<0.05)。模型组PTEN表达与假手术组无显著性差异(P=0.460),电针组较模型组显著降低(P<0.001)。结论电针可能通过抑制PTEN和增加GAP-43的表达促进脑缺血后神经再生和功能恢复。

Objective To investigate the effects of electroacupuncture （EA） on expression of phosphatase and tensin homology deleted on chromosome ten （PTEN） protein and growth associated protein-43 （GAP-43） in rats with Barrel cortex focal ischemia. Methods 24 adult male Sprague-Dawley rats were divided into sham group （n=8）, model group （n=8） and EA group （n=8）. The EA group accepted EA at Bai-hui （DU20） and Fengfu （DU16） acupoints 3 days after modeling, 30 min a time, once a day. They were assessed with Corner Test 1 day be-fore modeling, and 3 days, 7 days and 14 days after medication. The expression of GAP-43 and PTEN around infarct zone were detect with Western blotting 14 days after medication. Results The frequence of turn-right decreased in the EA group compared with that in the model group （P〈0.001） 14 days after modeling. The expression of GAP-43 increased in the model group compared with that in the sham group （P〈0.05）, and increased more in the EA group than in the model group （P〈0.05）. There was no significantly difference in PTEN expression be-tween the model group and the sham group （P=0.460）, and significantly decreased in the EA group （P〈0.001）. Conclusion EA may inhibit expression of PTEN protein and increase expression of GAP-43, which may be involved in nerve regeneration and functional recovery.