大鼠糖尿病性白内障晶状体中NADPH的实验研究

Analysis of lens NADPH activity in rat diabetic cataract

目的研究分析糖尿病大鼠晶状体内还原型辅酶Ⅱ(nicotinamide adenine dinucleotide 2'-phosphate reduced tetrasodium salt,NADPH)的变化及其原因。方法通过腹腔注射链脲佐菌素诱导1型糖尿病大鼠模型,待典型白内障形成时,检测晶状体内NADPH的含量以及6-磷酸葡萄糖脱氢酶(glucose-6-phosphate dehydrogenase,G6PD)的活性,并与对照组进行比较。结果同对照组透明晶状体相比,糖尿病性白内障(diabetic cataract,DC)晶状体内NADPH含量明显减少,G6PD活性显著降低。结论 G6PD活性的降低引起的NADPH含量减少以及伴随的氧化损伤加剧,与DC的发生密切相关。

Objective To identify lens nicotinamide adenine dinucleotide 2′-phosphate re-duced tetrasodium salt （ NADPH） activity changes in diabetic rats .Methods Type 1 diabetes was induced by abdominal streptozotocin injection in rats .When characteristic cataracts were present in the diabetic rats , the levels of NADPH and G6PD activity were measured and com-pared with those in controls .Results Lens NADPH was significantly decreased and G 6 PD ac-tivity was also decreased greatly in rat diabetic cataract （ DC） compared with those in transpar-ent lenses of controls .Conclusion The decreases of G6PD activity result in lower levels of NADPH and subsequent increase of oxidative damage , which is highly associated with DC formation.