NOD1在舌鳞状细胞癌组织中的表达及意义

Expression and clinical significance of NOD1 in tougue squamous cell carcinoma

目的探索NOD1在舌鳞状细胞癌组织中的表达及意义。方法采用免疫组织化学、RT-q PCR检测舌鳞状细胞癌组织和正常舌黏膜对照组中NOD1的表达,并分析其临床意义。应用SPSS13.0软件包,对各组mRNA的表达进行t检验。结果 NOD1蛋白表达于细胞质,舌鳞状细胞癌组织中NOD1蛋白表达率为60.0%(15/25),显著低于正常舌黏膜对照组表达率80.0%(20/25,P<0.05)。舌鳞状细胞癌组织NOD1 mRNA表达量为正常舌黏膜对照组(0.437±0.103)倍(P<0.05)。结论舌鳞状细胞癌组织中NOD1 mRNA和蛋白表达显著低于正常舌黏膜对照组,但需要在更大样本中验证这一结果,并开展功能学研究以揭示其潜在的生物学机制。

Objective To investigate expression and clinical significance of NOD1 in tougue squamous cell carcinoma （TSCC）. Methods Immunohistochemical staining and RT-qPCR were applied to evaluate NOD1 expression in TSCC and non-cancer tougue epi- thelial tissues. Its clinical significance would also be analyzed. T-test was performed to assess the statistical significance of mRNA ex- pression between the two groups using SPSS 13.0. Among many groups （ 〉 2 groups）, statistical significance was determined by two-way analysis of variance. Results NOD1 was expressed in the cytoplasm. NOD1 expression was significantly lower in TSCC tissues （60.0% , 15/25 ） than that in non-cancer tougue epithelial tissues （ 80.0% , 20/25 （ P 〈 0.05 ）. NOD1 messenger RNA expression in TSCC tissues was 0. 437 ± 0. 103 times as much as that in non-cancer tougue epithelial tissues （ P 〈 0.05 ）. Conclusions The RNA and protein expression of NOD1 are significantly lower in TSCC. However,further studies with functional evaluation of the NOD1 are warranted to replicate and extend the significance of the findings.