摘要
目的检测溃疡性结肠炎(UC)患者血清和结肠黏膜中低氧诱导因子-1α(HIF-1α)的含量,探讨其在UC发病机制中的调控作用及其与患者病情活动性和严重性的关系。方法采用酶联免疫吸附试验(ELISA)定量检测10名健康对照者、13例缓解期UC及47例活动期UC患者血清中HIF-1α含量,同时采用枸橼酸-微波-SP-免疫组织化学方法检测结肠黏膜中HIF-1α的表达情况,并评价其与活动期患者病情相关性。结果活动期UC患者血清HIF-1α含量(73.21±28.65)ng/L显著高于缓解期(44.54±14.75)ng/L和对照组(42.83±15.49)ng/L(P<0.05),且缓解期与对照组比较,差异无统计学意义(P>0.05),血清HIF-1α的表达与病情活动性、病情分型及内镜表现分级呈正相关;活动期UC结肠黏膜中HIF-1α表达阳性率(58.05±13.83)%显著高于缓解期(3.00±2.72)%和对照组(3.04±2.69)%(P<0.05),且缓解期与对照组相比,差异无统计学意义(P>0.05),结肠黏膜中HIF-1α的表达与病情活动性、病情分型及内镜表现分级也呈正相关。结论 HIF-1α作为一种转录因子可能在UC发病机制中有重要作用,并可能作为评价UC患者病情活动性及严重程度的良好指标。
Objective To investigate the expression of hypoxia inducible factor-1 alpha (HIF-1α) in serum and co- lon mucosa of ulcerative colitis (UC) patients, and explore its role in the pathogenesis of UC and its relationship with disease activity and severity. Methods Enzyme-linked immunosorbent assay (ELISA) was performed to detect the ex- pression of HIF-1α in 10 healthy controls, 13 cases of remission UC and 47 cases of active UC in serum, at the same time, Citrate-microwave-Streptavidin-HRP (SP) immunohistochemistry method was performed to detect the expression of HIF-1α in colon mucosa, and the active UC patients underwent the evaluation of disease severity. Results The expres- sion of HIF-1α in serum was significantly higher in active UC patients (73.21 ±28.65) ng/L than that in remission UC (44.54± 14.75) ng/L and control group (42.83±15.49) ng/L (P 〈 0.05) , but there was no significant difference between remission UC and control group (P 〉 0.05 ) , the level of HIF-1α in serum was positively correlated with dis- ease activity, disease severity and endoscopic grading. The positive rate of HIF-1α in colon mucosa of active UC patients (58.05 ±13.83)% was significantly higher than that in remission UC (3.00±2.72% ) and control group (3.04 ±2.69 ) % (P 〈 0.05 ), but there was no significant difference between remission UC and control group (P 〉 0.05 ), the level of HIF-1α in colon mucosa was positively correlated with disease activity, disease severity and endoscopic grading. Conclusion HIF-1α as a transcription factor may play an important role in the pathogenesis of UC, and may serve as a good index to evaluate disease activity and severity in patients with UC.
出处
《胃肠病学和肝病学杂志》
CAS
2014年第12期1433-1436,共4页
Chinese Journal of Gastroenterology and Hepatology