低氧诱导因子-1α在溃疡性结肠炎患者血清和结肠黏膜中的表达及意义

Expression and significance of hypoxia inducible factor-1α in serum and colon mucosa of patients with ulcerative colitis

目的检测溃疡性结肠炎(UC)患者血清和结肠黏膜中低氧诱导因子-1α(HIF-1α)的含量,探讨其在UC发病机制中的调控作用及其与患者病情活动性和严重性的关系。方法采用酶联免疫吸附试验(ELISA)定量检测10名健康对照者、13例缓解期UC及47例活动期UC患者血清中HIF-1α含量,同时采用枸橼酸-微波-SP-免疫组织化学方法检测结肠黏膜中HIF-1α的表达情况,并评价其与活动期患者病情相关性。结果活动期UC患者血清HIF-1α含量(73.21±28.65)ng/L显著高于缓解期(44.54±14.75)ng/L和对照组(42.83±15.49)ng/L(P<0.05),且缓解期与对照组比较,差异无统计学意义(P>0.05),血清HIF-1α的表达与病情活动性、病情分型及内镜表现分级呈正相关;活动期UC结肠黏膜中HIF-1α表达阳性率(58.05±13.83)%显著高于缓解期(3.00±2.72)%和对照组(3.04±2.69)%(P<0.05),且缓解期与对照组相比,差异无统计学意义(P>0.05),结肠黏膜中HIF-1α的表达与病情活动性、病情分型及内镜表现分级也呈正相关。结论 HIF-1α作为一种转录因子可能在UC发病机制中有重要作用,并可能作为评价UC患者病情活动性及严重程度的良好指标。

Objective To investigate the expression of hypoxia inducible factor-1 alpha （HIF-1α） in serum and co- lon mucosa of ulcerative colitis （UC） patients, and explore its role in the pathogenesis of UC and its relationship with disease activity and severity. Methods Enzyme-linked immunosorbent assay （ELISA） was performed to detect the ex- pression of HIF-1α in 10 healthy controls, 13 cases of remission UC and 47 cases of active UC in serum, at the same time, Citrate-microwave-Streptavidin-HRP （SP） immunohistochemistry method was performed to detect the expression of HIF-1α in colon mucosa, and the active UC patients underwent the evaluation of disease severity. Results The expres- sion of HIF-1α in serum was significantly higher in active UC patients （73.21 ±28.65） ng/L than that in remission UC （44.54± 14.75） ng/L and control group （42.83±15.49） ng/L （P 〈 0.05） , but there was no significant difference between remission UC and control group （P 〉 0.05 ） , the level of HIF-1α in serum was positively correlated with dis- ease activity, disease severity and endoscopic grading. The positive rate of HIF-1α in colon mucosa of active UC patients （58.05 ±13.83）% was significantly higher than that in remission UC （3.00±2.72% ） and control group （3.04 ±2.69 ） % （P 〈 0.05 ）, but there was no significant difference between remission UC and control group （P 〉 0.05 ）, the level of HIF-1α in colon mucosa was positively correlated with disease activity, disease severity and endoscopic grading. Conclusion HIF-1α as a transcription factor may play an important role in the pathogenesis of UC, and may serve as a good index to evaluate disease activity and severity in patients with UC.