^(18)F-FDG-PET/CT检测实验兔模型主动脉动脉粥样硬化及其炎性状态

Evaluation of atherosclerosis and inflammatory status of aortae in a rabbit model using ^(18)F-FDG-PET/CT

目的动脉粥样硬化在体动态评估可应用于临床预防心血管事件。本实验目的是监测F-18-氟脱氧葡萄糖(18F-FDG)在动脉粥样硬化不同阶段的摄取,研究利用正电子发射断层成像/计算机体层摄影技术(PET/CT)检测易损斑块的可行性。方法 22只雄性新西兰白兔随机分为2组:动脉粥样硬化组(A组,n=11)和动脉粥样硬化+他汀组(S组,n=11)。实验兔在第18周通过药物触发以诱导血栓形成。实验过程中的4个阶段分别进行PET/CT扫描:高胆固醇饮食前(基线阶段,n=6),第8周时(高胆固醇饮食中期,n=4),第18周时(触发实验前,即高胆固醇饮食结束阶段,n=22),药物触发实验后(触发实验后,n=15)。主动脉的18 F-FDG摄取值用最大标准化摄取值(SUVmax)和平均SUV值(SUVmean)表示。SUV值通过测量一系列7.5mm长度的动脉节段获得。结果SUVmean和SUVmax分别为基线阶段0.449±0.108和0.550±0.132,高胆固醇饮食中期0.694±0.117和0.754±0.129,触发实验前(高胆固醇饮食结束阶段)A组0.788±0.121和0.861±0.139,S组0.651±0.194和0.736±0.243。SUVmean和SUVmax在触发实验后血栓形成组分别为1.128±0.420和1.302±0.489,在无血栓形成组分别为0.774±0.159和0.859±0.191。22只实验兔中的10只经检测证实有血栓形成(45.5%):A组11只中的8只血栓形成(72.3%),S组11只中2只血栓形成(18.2%)(P<0.001)。结论 18F-FDG摄取值的定量分析可以检测动脉粥样硬化的炎性状态和易损斑块。PET/CT对预测临床中动脉粥样硬化患者的血栓形成事件有潜在应用价值。

Objective In vivo dynamic evaluation of atherosclerosis could be clinically significant in the prevention of car‐diovascular events .We aimed to monitor Fluorine‐18 fluorodeoxyglucose （18 F‐FDG） uptake in different stages of athero‐sclerosis ,and to investigate the feasibility of detecting vulnerable plaques using positron emission tomography /computed tomography （PET/CT） angiography .Methods 22 male NZW rabbits were divided into 2 groups：atherosclerosis group （group A ,n=11） and atherosclerosis ＋ statin group （group S ,n=11） .The rabbits underwent 2 pharmacological trig‐gerings to induce thrombus at the 18th week .In vivo PET/CT scans were performed on 4 time points ：before cholesterol diet （baseline ,n=6） ,at 8th week （the middle‐of‐feeding ,n= 4） ,at 18th week （the end‐of‐feeding ,n=22） and after triggering （post‐triggering ,n=15） .18 F‐FDG uptake by the aorta was expressed as maximal standardized uptake value （SUVmax） and mean SUV （SUVmean） .SUVs were measured on serial 7 .5 mm arterial segments .Results SUVmean and SUVmax were 0 .449 ± 0 .108 and 0 .550 ± 0 .132 at baseline ,0 .694 ± 0 .117 and 0 .754 ± 0 .129 at the middle‐of‐feed‐ing ,0 .788 ± 0 .121 and 0 .861 ± 0 .139 in group A ,and 0 .651 ± 0 .194 and 0 .736 ± 0 .243 in group S at the end‐of feeding before triggering .SUVmean and SUVmax were 1 .128 ± 0 .420 and 1 .302 ± 0 .489 in thrombosis group ,0 .774 ± 0 .159 and 0 .859 ± 0 .191 in non‐thrombosis group after triggering .Thrombi were identified in 10 of 22 rabbits （45 .5% ）：8 of 11 （72 .3% ） in group A ,and 2 of 11 （18 .2% ） in group S （ P〈0 .001） .Conclusion The inflammatory status of atheroscle‐rosis and vulnerable plaque can be detected by quantitative analysis of 18 F‐FDG uptake .PET/CT may be used for predic‐ting thrombosis events in patients with atherosclerotic disease .