亚砷酸钠对胰岛细胞中p53、mdm2和Kras基因及蛋白表达的影响

Effect of sodium arsenite on the gene and protein expression of p53,mdm2 and Kras in islet cells

目的探讨砷对大鼠胰岛β细胞(INS-1)p53、mdm2和Kras基因和蛋白表达的影响。方法荧光实时定量PCR法检测亚砷酸钠对大鼠胰岛β细胞中野生型p53(Tp53)、mdm2和Kras基因表达的影响,Western blot检测亚砷酸钠对大鼠胰岛β细胞突变型p53和mdm2蛋白表达的影响。结果亚砷酸钠作用于INS-1细胞后,Tp53基因水平降低,mdm2、Kras基因水平升高;突变型p53和mdm2蛋白表达增加。结论亚砷酸钠可使INS-1细胞中抑癌基因Tp53向突变型p53转变,癌基因mdm2和Kras的水平升高。

Objective To investigate the effect of sodium arsenite on p53, mdm2 and Kras expressions in islet βcells （INS-1） of rat.Methods The levels of wild type p53 （Tp53）, mdm2 and Kras in sodium arsenite-treated rat islet βcells were detected by real-time PCR.The expression of mutant p53 and mdm2 protein were detected by western blot. Results After treatment with sodium arsenite, the level of Tp53 decreased, but those of mdm2、Kras increased.The protein expressions of mutant p53 and mdm2 increased.Conclusion Sodium arsenite could induce the transformation of Tp53 to mutant p53, and increase the level of mdm2 and Kras in INS-1 cells.