摘要
目的:观察身痛逐瘀胶囊对冰醋酸致痛小鼠脑组织c-fos蛋白、GFAP蛋白和β-EP蛋白表达的影响。方法:以免疫组织化学方法观察冰醋酸致痛小鼠脑组织c-fos蛋白、GFAP蛋白和β-EP蛋白表达。结果:身痛逐瘀胶囊1.64g/kg、0.82g/kg、0.41g/kg可以下调实验小鼠脑组织中c-fos蛋白的表达;身痛逐瘀胶囊1.64g/kg、0.82g/kg可以下调实验小鼠脑组织中GFAP蛋白的表达,上调β-EP蛋白的表达。结论:身痛逐瘀胶囊的镇痛机制与c-fos蛋白、GFAP蛋白的表达下调、β-EP蛋白的表达上调有关。
Objective： To investigate the effects of Shentongzhuyu capsules on c-fos,glial fibrillary acidic protein（ GFAP）,and β-endorphin（ β-EP） in brain of pain model of mice which induced by acetic acid. Methods： Acetic acid writhing method was used to study analgesic effect of Shentongzhuyu capsules. The expressions of c-fos,GFAP and β-EP protein were detected by immunohistochemistry. Results： In comparison with the model controls,c-fos in brain tissue of pain model of mice was significantly down-regulated by Shentongzhuyu capsule（ 1. 64 g / kg,0.82 g / kg,0. 41 g / kg）,at the same time,GFAP in brain tissue of pain model of mice was significantly down-regulated and β-EP in brain tissue of pain model of mice was significantly up-regulated. by Shentongzhuyu capsule（ 1. 64 g / kg,0. 82 g / kg）. Conclusion： The mechanisms of antinociceptive effect shown by Shengtongzhuyu capsule may be related to regulating the expression of c-fos protein,GFAP protein,β-EP protein.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2014年第5期5-8,共4页
Pharmacology and Clinics of Chinese Materia Medica
