高糖和高脂饮食对小鼠肥胖和内脏脂肪的影响

Effect of High-fat or High-glucose Diet on Obesity and Visceral Adipose Tissue in Mice

目的探讨高脂和高糖饮食对小鼠肥胖和内脏脂肪的不同影响。方法将4周龄C57BL/6小鼠按随机数字表法分为正常饮食组、高脂饮食组和高糖饮食组,喂养至20周龄,比较各组体重、附睾旁脂肪重量、瘦素、脂肪肝程度、附睾旁脂肪组织巨噬细胞及其亚型的浸润和单核细胞趋化蛋白-1 mRNA的表达。结果 20周时高脂饮食组小鼠体重、附睾旁脂肪重量、瘦素水平较正常饮食组均显著升高(P均<0.05),油红O染色显示高脂饮食组肝脏脂肪浸润明显。20周时高糖饮食组在体重、附睾旁脂肪重量、瘦素水平与对照组相比差异均无统计学意义(P均>0.05)。高脂饮食组小鼠脂肪内巨噬细胞的浸润增加,且M2的比例较高糖饮食组显著降低(P<0.05)。高脂饮食组小鼠附睾旁脂肪中单核细胞趋化蛋白-1 mRNA的表达较正常饮食组显著上调(P<0.05),而高糖饮食组与正常饮食组相比差异无统计学意义(P>0.05)。结论高脂饮食,而不是高糖饮食,可导致肥胖和内脏脂肪组织内巨噬细胞的炎症浸润。

Objective To investigate the effect of high-fat or high-glucose diet on obesity and visceral adipose tissue in C57BL/6 mice.Methods Four-week-old C57BL/6 mice were allocated into normal diet group,high-fat diet group,and high-glucose diet group according to the random number table until 20 weeks old.Body weight,epididymal adipose tissue weight,blood leptin,fat infiltration in liver,M1/M2 macrophage subtypes,and monocyte chemoattractant protein-1 mRNA in epididymal adipose tissues were measured.Results Compared with normal diet group,body weight,epididymal adipose tissue weight,and leptin concentration in high fat diet group at 20 weeks were significantly increased （P 〈 0.05）,and oil red O staining showed more prominent adipocyte infiltration in liver in high-fat diet group than those in normal diet and high-glucose diet group.However,no apparent differences were seen in high-glucose diet group at 20 weeks in terms of body weight,epididymal adipose tissue weight and leptin concentration.In high-fat diet group,the macrophages infiltration in epididymal adipose tissue increased with time and the percentage of M2 macrophage decreased in highfat diet group than that in high-glucose diet group （P 〈 0.05）.Compared with normal diet group,monocyte chemoattractant protein-1 mRNA expression increased significantly in high-fat diet group （P 〈 0.05）.In highglucose group,however,no significant differences were discerned （P 〉 0.05）.Conclusion High-fat diet,rather than 60% high glucose diet,will lead to obesity and macrophage infiltration in adipose tissues.