大黄鱼QM-like(PcQM)蛋白生物信息学分析

Bioinformatics analysis of QM-like(PcQM) protein from Pseudosciaena crocea

[目的]通过分析PcQM蛋白质的结构,预测其高级结构和功能,为进一步研究PcQM蛋白提供理论依据。[方法]利用Blast P和相关分析蛋白质理化性质与结构的软件,对PcQM蛋白进行结构与功能预测分析。[结果]PcQM蛋白不存在N端信号肽,存在多个酶切位点,无跨膜结构,定位在细胞质中。不存在二硫键,该蛋白含26.05%α-螺旋结构(H),20.93%β-折叠结构(E),53.02%无规则卷曲(L)。最后,通过Swiss-Model程序预测了PcQM蛋白的三级结构。[结论]较全面地分析和预测了PcQM蛋白的理化性质、二级结构和三级结构等。为深入分析PcQM在大黄鱼(Larimichthys crocea)体内免疫调节功能研究,提供了理论依据。

[ Objective ] The purpose of this study was to learn more function of PcQM protein by analyzing the AA sequence and predicting the structure of it. [ Methods] Some related bioinformatics analysis software or system on interuet such as Blast P, PredictProtein, Swiss - Model,was used to analyze the structure and prediction the structure and function of PcQM protein. [ Results] The results showed that Pc- QM protein had many restriction enzyme sites. There was no signal peptide on N -terminal and no transmembrane structure. It was located in the cytoplasm. There were 26. 05% ot - helical （H） ,20. 93% 13 - pleated sheet （E） and 53.02% random coil （L） predicted by Pre- dictProtein. The tertiary structure was predicted by Swiss - Model. [ Conclusion] The study has analyzed the sequence of PcQM protein and predicted the structure of it,which would lay a foundation for further learning the relationship between structure and function of the protein.