异柠檬酸裂解酶肽类抑制剂筛选及活性检测

Screening and detecting activity of isocitrate lyase peptide inhibitor

[目的]通过噬菌体的肽库筛选并合成具有抑制ICL活性的多肽。[方法]以ICL为基础,利用Discovery Studio 2.1中ligentfit模块,将筛选出多肽与优化后ICL进行分子对接。合成并进行生物活性检测。[结果]通过噬菌体肽库筛选得到6条的七肽成功和ICL对接。合成后的6条七肽质谱检测结果均正确。对6条七肽进行体外生物活性检测,对ICL酶的活性均明显具有抑制的作用。其中3条七肽抑制率超过了70%。[结论]利用虚拟筛选优化了ICL抑制剂的筛选过程,筛选并合成ICL抑制剂,抑制率为75%、64%、66%、77%、78%、67%,为抗结核多肽药物研发提供基础。

[ Objective ] Using the phage peptide library to screen and synthesize peptides for inhibiting ICL activity. [ Methods ] Based on ICL,via the Discovery Studio 2. 1 simulate, peptides were docked with ICL. The peotides were synthesized and their biological activity were detected. [ Results] 6 heptapeptide were gotten by phage peptide library screening and docked with ICL successfully. The biological activity detecting showed that they all could inhibit the activity of ICL significantly. Three of them were over 70%. [ Conclusion] We optimized the screening of ICL inhibitor, synthesized ICL inhibitor according to the screening result, inhibitor rate 75%, 64%, 66%, 77%, 78% ,67%, and provided experiment data for developing new anti -TB drugs.