叉头转录因子3在鼻息肉中的表达及意义

Expression of Foxp3 and its significance in nasal polyps

目的探讨叉头转录因子3(forkheadtranscription factor P3,Foxp3)和嗜酸性粒细胞(EOS)及肥大细胞在人鼻息肉组织、鼻息肉周围组织和慢性鼻窦炎筛窦黏膜中的表达情况。方法采用免疫组化Elivision法检测Foxp3在20例鼻息肉组织、15例息肉周围组织、20例筛窦黏膜组织和10例正常下鼻甲黏膜组织中的表达,同时采用HE染色、肥大细胞特殊染色计数各组织中EOS和肥大细胞的浸润程度。结果鼻息肉组织中EOS、肥大细胞数均明显高于其他3组,而Foxp3在鼻息肉中则呈低表达,且差异具有统计学意义(P<0.05),EOS、Fox3的表达4组比较具有统计学意义(P<0.01),两两比较除在息肉周围组织与鼻窦炎组织无统计学意义(P>0.05)外,其余两两比较均具有统计学意义(P<0.05)。肥大细胞的表达4组比较具有统计学意义(P<0.01),除息肉组织外,其余3组比较差异无统计学意义(P>0.05),且Foxp3阳性细胞数与EOS浸润数在各组中均呈负相关(P<0.0 1)。结论鼻息肉是以EOS浸润为主要特征,Foxp3的下调引起的抑制功能低下可能是引起鼻息肉发病的原因之一,肥大细胞在鼻息肉的发生发展过程中发挥重要作用。

Objective To investigate the expression of forkhead family transcription factor 3 （Foxp3）, eosinophils （Eos） and mast cells in human nasal polyps, tissues around polyp and ethmoidal mucosa of chronic sinusitis. Methods Immunohistochemical method was used for detecting the expression of Foxp3 in nasal polyp （ n = 20 ） , tissue around polyp （ n = 15 ） , ethmoidal mucosa of chronic sinusitis （ n = 20 ） and mucosa of normal inferior turbinate （ n = 10 ）. HE staining and mast cellspecific staining were adopted to determine the infiltration of Eos and mast cells in all these specimens. Results The counts of Eos and mast cell in polyp group were significantly higher than those of the other 3 groups （ P 〈 0.05 ） , while the expression level of Foxp3 in polyp group was lower than those of the other 3 groups （ P 〈 0. 05 ） . The differences of expression of EOS, Fox3 among 4 groups were statistically significant （ P 〈 0.01 ）. Pairwise comparisons showed significant differences except that between the tissue around polyp and the mucosa of chronic sinusitis （ P 〉 0.05 ）. Mast cell counts among 4 groups were statistically significant （ P 〈 0.01 ） , and pairwise comparisons showed insignificant differences in groups except the nasal polyp （ P 〈 0.05 ）. The Eos counts were negatively correlated with the amount of Foxp3 positive cells in all 4 groups （ all P 〈 0.01 ）. Conclusion Nasal polyp is characterized by Eos accumulation. Inhibition dysfunction caused by down regulation of Foxp3 expression maybe one factor in pathogenic mechanisms of nasal polyp. Mast cell plays an important role in the development and progression of nasal polyps.