TRPV6、Ki-67在溃疡性结肠炎、结肠癌中的表达及意义

TRPV6 and Ki-67 expression in ulcerative colitis and colon carcinoma

目的:旨在探索TRPV6、Ki-67在溃疡性结肠炎(ulcerative colitis,UC)、结肠癌组织中的表达情况以及与细胞增殖、恶变之间的联系.方法:收集遵义医学院附属医院行结肠镜下取病理活检的结肠组织标本,结合其临床资料,共获取UC标本20例,结肠癌组织20例,肠镜检查未见异常的肠黏膜组织20例.通过免疫组织化学染色,观察UC、结肠癌及正常结肠组织中TRPV6、Ki-67的表达情况.结果:(1)在正常结肠黏膜组织、UC以及结肠癌中均有TRPV6的表达,但TRPV6在UC和结肠癌组中的表达率显著高于正常黏膜组(P<0.05),其阳性表达部位位于胞浆和/或胞膜;Ki-67的阳性率呈递增趋势,其在UC和结肠癌中的阳性表达率显著高于正常黏膜组(P<0.05),其阳性表达部位位于胞核;(2)TRPV6、Ki-67在UC和结肠癌中的表达呈正相关(r=0.48,P<0.05;r=0.69,P<0.05).结论:TRPV6、Ki-67在人UC、结肠癌以及正常结肠等组织中有不同程度的表达,TRPV6在UC和结肠癌组中的表达率均显著高于正常黏膜组.推测TRPV6可能在UC、结肠癌的发生、发展过程中扮演着重要的角色.

AIM： To investigate the TRPV6 and Ki-67 expression in ulcerative colitis and colon cancer. METHODS： The expression of TRPV6 and Ki-67 was examined by immunohistochemistry in 20 ulcerative colitis tissues, 20 colon cancer tissues and 20 normal colonic mucosal tissues. RESULTS： TRPV6 expression gradually increased from normal colon tissue to ulcerative colitis tissue and colonic cancer. The positive rates of TRPV6 in ulcerative colitis and colon cancer were significantly higher than that in the normal colon tissue （P 〈 0.05）. TRPV6 expression was located in the cytoplasm and/or cell membrane. The positive rates of Ki-67 in ulcerative colitis and colon cancer were significantly higher than that in the normal colon tissue （P 〈 0.05）. Ki-67 expression was located in the nucleus. The expression of TRPV6 had a positive correlation with that of Ki-67 in ulcerative colitis and colonic cancer （r = 0.48, P 〈 0.05; r = 0.69, P 〈 0.05）. CONCLUSION： The expression of TRPV6 and Ki-67 differs among ulcerative colitis, colon cancer and normal colon. The expression of TRPV6 in ulcerative colitis and colon cancer is significantly higher than that normal colon tissue, suggesting that TRPV6 may be related to the pathogenesis and development of ulcerative colitis and colon cancer.