离体皮肤氧耗量测定与微机数据分析系统

Measurement of oxygen consumption in split-thickness skin and the computer analysis of data

目的比较长短氧传感器常规手工计算法和微机数据分析法测定皮片氧耗活力的效果。方法将Wistar鼠薄皮片分别切成直径为15mm和6mm的小皮片,一部分皮片立即测定氧耗量和另一部分皮片液氮冻存。两种氧传感器对每块皮片各测定两次,然后按常规法和微机法计算测定皮片1min的氧耗量。结果两种氧传感器常规法和微机法测定新鲜皮片的氧耗量各组内均无显著性差异(P>0.05);但长氧传感器测定的氧耗量比短氧传感器测得的氧耗量显著高(P<0.05)。两种氧传感器微机法测定皮片的氧耗量比常规法测定皮片的氧耗量显著高(P<0.05)。长氧传感器和微机法测定冻存皮片的氧耗量比新鲜皮片氧耗量显著低(P<0.01)。结论结果提示长氧传感器比短氧传感器测定皮片氧耗量更高、用皮量更少,而微机法比常规法更加简便可靠。

Objective To compare the differences between long and short oxygen sensor with routine manual assay and microcomputer analysis of data regarding to the oxygen consumption in split thickness skin. Methods The split thickness skin of Wistar rats were cut into pieces measuring 6 mm and 15 mm in diameter.The rate of oxygen consumption was measured immediately for a portion of the skin, and the another portion was frozen in liquid nitrogen.Two kinds of oxygen sensors were used to measured for two times for each piece of the split thickness skin, and then the oxygen consumption within 1 minute for split thickness skin was measured by routine and microcomputer analysis.Results There was no significant difference in the rate of the oxygen consumption in fresh skin measured with two kinds of oxygen sensor by routine and microcomputer analysis (P >0.05 ) within each groups. The rate of oxygen consumption of long oxygen sensor was obviously higher than that of short oxygen sensor (P< 0.05). The rates of oxygen consumption measured by two kinds of oxygen sensors with microcomputer analysis were obviously higher than that with the routine method (P< 0.01). The oxygen consumption in frozen skin measured by long oxygen sensor with microcomputer analysis were significantly lower than that in fresh skin (P< 0.01). Conclusions These results suggest that oxygen consumption in the split thickness skin measured with long oxygen sensor appeare to be higher with less skin compared with short oxygen sensor.Microcomputer analysis is a simple and reliable method compared with the routine assay.