高效液相色谱法测定潮州花生糖中的黄曲霉毒素B_1

Determination of Aflatoxin B_1 in Chaozhou Peanut Brittle by High Performance Liquid Chromatography

建立免疫亲和层析净化-高效液相色谱法测定潮州花生糖中的黄曲霉毒素B1的检测方法。样品用甲醇-水(7+3)提取,定性滤纸和玻璃纤维滤纸2次过滤,过免疫亲和柱净化;洗脱液氮气吹干后加正己烷和三氟乙酸衍生,以水-乙腈(85+15)溶解,用高效液相色谱仪带荧光检测器检测。方法在0.002 5 ng/μL^0.100 ng/μL的范围内呈良好的线性关系,加标平均回收率为85.3%~92.9%,试验的RSD为0.61%~1.08%。该方法通过改进柱前衍生化的条件和优化仪器分析条件,实验结果重现性好,回收率高,结果准确,为黄曲霉毒素B1的检测提供了准确可靠的方法。

A method was developed for the determination of Aflatoxin B1 in Chaozhou peanut brittle by Immunoaffinity chromatography and High Performance Liquid Chromatography. Samples extracted with methanol- water （7＋3）, filtered with qualitative filter paper and glass fiber filter paper, purified by immunoaffinity column;Eluate was dried with nitrogen, and derived by n-hexane and trifluoroacetic acid, dissolved with water-acetonitrile （85＋15） and detected by high performance liquid chromatography with fluorescence detection. The calibration curves are linear between 0.002 5 ng/μL and 0.100 ng/μL. The rate of average recovery of aflatoxin B 1 was 85.3%-92.9%,and RSDs ranged from 0.61%to 1.08%. In this paper,optimized the condition of HPLC and the conditions of pre-column derivatization, the method has the advantages of simplicity,rapidity,precision,good reproducibility and high recovery,so that it could be used for the determination of aflatoxin B1, and it can meet the need of measuring in food safety control.