局部注射VEGF对大鼠正畸牙牙齿移动和体质量的影响

Effect of recombinant human vascular endothelial growth factor on orthodontic tooth movement andweight in rats

目的研究局部应用外源性重组人血管内皮生长因子(rhVEGF)对大鼠正畸牙牙齿移动距离和体质量的影响。方法建立SD大鼠正畸牙近中移动动物模型,将30只模型大鼠按rhVEGF注射剂量不同随机分为0(对照组)、1ng、5ng、10ng、15ng和20ng组,每组5只,将不同剂量rhVEGF每3d注射于模型大鼠左上颌第一磨牙颊侧牙龈黏膜下,10d后处死,测量牙齿移动距离并监测体质量的改变。结果注射rhVEGF剂量低于5ng时牙齿移动距离较小,随剂量增大而增加,5ng时到达高峰值,与对照组比较差异有统计学意义(P<0.05);随后牙齿移动距离随剂量增加而减小;其余剂量组与对照组比较差异均无统计学意义(P>0.05)。各组大鼠处死时体质量均比实验前轻,但各组间差异无统计学意义(P>0.05)。结论一定剂量范围内的外源性rhVEGF可加快正畸牙移动,局部短时间内应用外源性rhVEGF对正畸模型大鼠体质量无明显影响。

Objective To observe the effect of recombinant human vascular endothelial growth factor （ rhVEGF） on orthodontic tooth movement and weight in rats .Methods Model for orthodontic tooth movement was established in 30 SD rats ,which were divided into six groups （n=5 ,each group） and injected respectively with different doses of rhVEGF（0（the control group） ,1 ,5 10 ,15 ,20ng） in‐to the palatal submucosal area adjacent to the left upper first molar once per 3 days .The animals were sacrificed on tenth day ,and the distances of tooth movement and animal weight were measured .Results The maximum tooth movement was observed in the 5ng group .The weight changes of all animal were not obviously .Conclusion Within certain limits ,exogenous rhVEGF can accelerate the orthodontic tooth movement ,and has no effect body weight in a short period of time .