HIV和HCV合并感染对患者外周血中A3GmRNA及干扰素α表达的影响

Impact of HIV/HCV co-infection on peripheral expression of A3G mRNA and interferon-α

目的：研究HIV和HCV（HIV／HCV）感染对患者外周血单个核细胞（PBMC）中A3G mRNA表达及外周血中内源性干扰素（ IFN）-α水平的影响。方法以未经抗病毒治疗的慢性丙型肝炎患者（ HCV单一感染组，43例）、艾滋病患者（ HIV单一感染组， CD4＋T 淋巴细胞＜200个／μL，45例）和HIV／HCV合并感染组（ CD4^＋T淋巴细胞＜200个／μL，45例）为研究对象，并以23名我院门诊健康体检者作为对照组。采用实时荧光定量PCR法检测A3G mRNA，酶联免疫吸附试验（ ELISA）检测血浆中内源性IFN-α的表达量。组间比较采用秩和检验，相关性分析用Spearman秩相关分析。结果 HIV单一感染组、HIV／HCV合并感染组、HCV单一感染组和健康对照组PBMC中A3G mRNA的表达量分别为4.89（0.59），4.85（0.71），3.89（1.08）和3.69（0.81）lg拷贝／mL。其中，HIV单一感染组和HIV／HCV合并感染组PBMC中A3G mRNA的表达量均明显高于健康对照组（ Z＝－6.306和－6.280，P＜0.01）和HCV单一感染组（Z＝－7.358和－7.275，P＜0.01）。 HIV单一感染组、HIV／HCV合并感染组、HCV单一感染组和健康对照组血浆中 IFN-α表达量分别为2.79（1.25），2.05（1.29），2.32（1.84）和2.16（2.19） pg／mL，其中 HIV 单一感染组血浆中 IFN-α表达量稍高于HIV／HCV合并感染组（Z＝－2.332，P＜0.05），其他各组血浆中IFN-α表达量比较差异均无统计学意义（P值均＞0.05）。血浆中 IFN-α水平与 A3G mRNA 表达水平未见明显相关性（rs ＝0.04，P ＞0.05），A3G mRNA 及 IFN-α表达量与HIV 及 HCV RNA 载量亦无明显相关性（P 值均＞0.05）。结论 HIV感染者PBMC高表达A3G，合并HCV感染对艾滋病患者的A3G表达无明显影响；A3G mRNA与IFN-α无明显相关性，且对HIV、HCV RNA载量的影响并不明显。

Objective To study the impact of HIV and hepatitis C virus （ HCV ） infection on peripheral expression of antiviral protein A3G and plasma IFN-αlevels.Methods Untreated patients with chronic hepatitis C（HCV infection group, n=43）, AIDS（HIV infection group, CD4 ＋T〈200 cells/μL, n=45） and HIV/HCV co-infection （CD4 ＋T〈200 cells/μL, n=45） were recruited in the study, and 23 healthy subjects were also enrolled as controls.A3G mRNA in peripheral blood mononuclear cells （PBMC） was measured by quantificational real-time PCR, and plasma IFN-αlevel was determined by enzyme linked immunosorbent assay （ELISA）.Rank-sum test and Spearman rank correlation analysis were performed. Results A3G mRNA levels in HIV infected group, HIV/HCV co-infected group, HCV infected group and＆amp;nbsp;healthy control group were 4.89 （0.59）, 4.85 （0.71）, 3.89 （1.08） and 3.69 （0.81） lg copies/mL, respectively.A3G mRNA levels in HIV infected group and HIV/HCV co-infected group were much higher than those in healthy control group （Z=-6.306 and -6.280, P〈0.01） and HCV infected group （Z=-7.358 and -7.275, P〉0.01）.Plasma IFN-αlevels in HIV infected group, HIV/HCV co-infected group, HCV infected group and healthy control group were 2.79 （1.25）, 2.05 （1.29）, 2.32 （1.84） and 2.16 （2.19） pg/mL, respectively.Plasma level of IFN-αin HIV infected group was higher than that in the HIV/HCV co-infected group （Z=-2.332, P〉0.05）, but no significant difference was observed among other groups （all P〈0.05）.There was no significant correlation between plasma IFN-αlevel and A3G mRNA expression （rs =0.04, P〈0.05）, and the levels of A3G mRNA and IFN-αshowed no correlation with HIV RNA and HCV RNA （all P〉0.05）.Conclusions A3G is highly expressed in PBMCs from HIV infected patients, and it may not be affected by the infection of HCV.A3G mRNA is not closely correlated with IFN-α, and it has not significant influence on HIV RNA and HCV RNA replication.