漳州水仙的组织培养技术

Tissue Culture of Zhangzhou Narcissus

以漳州水仙为材料,研究了漳州水仙的组织培养技术,优选出不定芽再生与生根的最佳培养基,结果表明:鳞茎盘与愈伤组织分化不定芽的最优培养基都为MS+6-BA 10 mg·L-1+NAA 1.0 mg·L-1+蔗糖30 g·L-1+植物凝胶3 g·L-1;生根的最佳培养基为1/2 MS+NAA 0.1 mg·L-1+IBA 0.1mg·L-1+蔗糖15 g·L-1的液体培养基。

Zhangzhou Narcissus（ Narcissus tazetta var. chinensis）,one of the 10 famous traditional flowers in China,was tissue cultured with bulb-scale and leaf segments as explants on the callus inducing and rooting mediums supplemented with different auxin combinations. Calli were induced from both segments on the mediums but no adventitious buds were developed from callus culture of bulb leaf segment. The optimal medium for adventitious bud formation from calli induced from bulb scale segments was MS ＋ 6-BA 10 mg·L- 1＋ NAA 1. 0mg·L- 1＋ sucrose 30 g·L- 1＋ phytagel 3 g·L- 1,and the optimal rooting medium was 1 /2 MS ＋ NAA 0. 1 mg·L- 1＋ IBA 0. 1 mg·L- 1＋ sucrose30 g·L- 1＋ phytagel 0 g·L- 1.