紫外-可见分光光度法测定沉香中色酮成分的含量

Determination of Chromones Content in Agarwood by Using UV Spectrophotometry

建立沉香中色酮类化合物总含量测定方法,首次利用紫外分光光度计对11批沉香乙醇提取物进行测定。结果表明,该方法精密度、重复性、稳定性和加样回收率均达到要求,11批沉香样品中色酮含量范围为4.5%~27.1%,根据色酮成分百分含量,将其分成3个等级。该方法简便快速,为沉香中色酮类成分含量测定和质量评价提供了科学方法。

A new method for quantitative determination of chromones in Agarwood（ Aquilaria sinensis） was established by using ultraviolet（ UV） spectrophotometry to detect ethanol extracts of 11 Agarwood samples. This method is precise,repeatable and stable and has a good recovery rate for the amount added. The eleven Agarwood samples were divided into three groups based on their chromones content of from 4. 5% to 27. 1%. The method is simple and quick,and provides a scientific method for determination of chromones content and for quality evaluation of Agarwood.