内源性缩醛磷脂对大鼠脑缺血损伤的保护作用

The protection of endogenous plasmalogen on ischemic brain injury in rats

目的探察证实内源性缩醛磷脂对大鼠缺血性脑损伤的保护作用。方法采用行为学探测、经典组化和免疫组化技术对模型大鼠的运动和学习记忆以及纹状体组织病理变化进行形态学探察,实验数据采用SPSS软件统计学处理。结果 1运动行为学探测显示缺血大鼠通过平衡木所需时间明显延长(P<0.05),缩醛磷脂干预的大鼠比缺血大鼠所需时间缩短(P<0.05);握力测试中,缺血大鼠抓握时间缩短(P<0.05),治疗组大鼠抓握时间比缺血组延长(P<0.05)。Morris水迷宫实验中,缩醛磷脂干预的大鼠逃避潜伏期与缺血组相比明显缩短(P<0.05),空间探索经过平台次数明显多于缺血组(P<0.05)。2组织学与免疫组织化学探察显示,缺血大鼠纹状体的背外侧区出现明显的损伤区域,损伤区域中神经元几乎完全丢失,而缩醛磷脂干预组的大鼠则有少数体积较小的神经元幸存。结论内源性缩醛磷脂对大鼠脑缺血损伤有保护作用。

Objective To confirm the protection effect of plasmalogen against cerebral ischemia. Methods Behavioral tests, histochemical and immunohistochemical stainings were used to evaluate the changes of motor behavior, cognitive function and striatal histology. Experimental data was analyzed by SPSS software. Results 1 In behavioral tests, compared with the cerebral ischemic rats, the rats intraperitoneally injected with myo-inositol significantly took less time to complete the task in the balance-beam task and lasted for longer in the grip-strength task（P〈0.05, P〈0.05）. Similarly, the myo-inositol-treated rats had significantly shorter escaping latency and passed over the platform more times in probe trail in Morris water maze test than ischemic rats（P〈0.05, P〈0.05）. 2Histochemical and immunohistochemical stainings revealed that a localized lesion appeared in the dorsal striatum after middle cerebral artery occlusion（MCAO）. Neurons almost totally lost in the center of lesion, but a few smallsize neurons were survived only after the myo-inositol treatment. Conclusion Intraperitoneal injection with myo-inositol increased the level of endogenous plasmalogen to against cerebral ischemia and to protect neurons.