弯锥香茶菜地下根茎的化学成分研究

Study on the chemical constituents from the rhizomes of Isodon loxothyrsa

目的研究弯锥香茶菜Isodon loxothyrsa地下根茎的化学成分。方法采用硅胶柱层析、RP-18、Sephadex LH-20等进行分离纯化,通过波谱法进行结构鉴定。结果从弯锥香茶菜的地下根茎70%丙酮提取物的乙酸乙酯部位中分得10个化合物,分别鉴定为齐墩果酸(Ⅰ)、蒲公英甾醇(Ⅱ)、β-谷甾醇(Ⅲ)、棕榈酸(Ⅳ)、6,12,15-三羟基-5,8,11,13-松香四烯-7-酮(Ⅴ)、咖啡酸甲酯(Ⅵ)、胡萝卜苷(Ⅶ)、迷迭香酸甲酯(Ⅷ)、2α-羟基乌索酸(Ⅸ)、3-(3',4'-二羟基苯基)-(2R)-乳酸甲酯(X)。结论所有的化合物均是首次从该植物中分离得到。

OBJECTIVE To study the chemical pattern from the underground part from Isodon loxothyrsa. METHODS The constit- uents were isolated by silica gel column chromatography, RP - 18 and sephadex LH -20 etc. , and their structures were identified on the basis of spectral data and physicochemical properties. RESULTS Ten compounds were obtained from ethyl acetate fraction of the 70% acetone extract of the rhizomes and identified as oleanolic acid（ Ⅰ ） ,taraxasterol（ Ⅱ ） , β - sitosterol（ Ⅲ ） ,palmitic acid（ Ⅳ ） ,6, 12,15 - trihydroxy - 5,8,11,13 - abietatetraen - 7 - one （ Ⅴ ）, methyl caffeate （ Ⅵ ） , daucosterol （ Ⅶ ）, rosmarinic acid methyl ester （ Ⅷ）, 2α - hydroxyursolic acid （ Ⅸ ）, and 3 - （ 3＇, 4＇ - dihydroxyphenyl） - （2R） - lactic acid methyl ester （ Ⅹ ）. CONCLUSION These compounds are obtained from this plant for the first time.