健脾消渴方对2型糖尿病大鼠胰岛细胞分泌功能的影响

Effect of Jianpi Xiaoke Formula on the Secretion of Islet Cells of Type 2 Diabetes Mellitus Rats

目的探讨健脾消渴方对2型糖尿病(T2DM)的作用及机制。方法将46只大鼠随机分为正常组(10只)和造模组(36只)。正常组常规饲料喂养,造模组大鼠高糖高脂饲料喂养。6周后,造模组大鼠一次性腹腔内注射链脲佐菌素(30 mg/kg)建立T2DM模型。36只造模成功大鼠随机分为模型组、优降糖组、中药组,每组12只。优降糖组给予优降糖混悬液0.27 mg/(kg·d)灌胃,中药组给予健脾消渴方水煎液6.36 g/(kg·d)灌胃,正常组和模型组给予等量生理盐水。6周后观察大鼠体重并检测各组大鼠空腹血糖(FBG)、空腹胰岛素(FINS)、胰高血糖素(GC),计算胰岛素抵抗指数(HOMA-IR)及胰岛素分泌指数(HOMA-β),免疫荧光分析胰岛β、α细胞在胰腺中的分布和定位。结果优降糖组和中药组给药后FBG、体重较本组给药前显著下降,并且FBG较同时间点的模型组显著降低(P<0.05或P<0.01)。与模型组比较,优降糖组和中药组GC水平下降明显(P<0.01),FINS、HOMA-β水平升高(P<0.05或P<0.01),优降糖组FINS、HOMA-IR、HOMA-β水平明显高于中药组(P<0.05)。免疫荧光显示中药组胰岛β细胞数量较模型组和优降糖组明显增多,胰岛α细胞数量减少。结论健脾消渴方可升高T2DM大鼠FINS、降低GC及改善胰岛素抵抗,调节胰岛α、β细胞在胰腺分布和数量,这可能是其作用机制之一。

Objective To analyze the effect and possible mechanism of Jianpi Xiaoke Formula（ JPXKF） on type 2 diabetes mellitus（ T2DM） rats. Methods Totally 46 rats were randomly assigned to normal group（ n =10） and model-making group（ n = 36）. The normal group was given regular forage,while the model group was fed with high-glucose and high-fat forage. After 6 weeks,the model-making group were intraperitoneally injected with streptozotocin（ 30 mg / kg） to make T2 MD models,and the 36 successfully made models rats were randomly divided to Model group,Glibenclamide group,TCM group,with 12 rats in each. The Glibenclamide group and TCM group were given glibenclamide suspension 0. 27 mg /（ kg·d） and JPXKF decoction 6. 36 g /（ kg·d） by gavage respectively,while the normal group and Model group were given the same amount of normal saline. After six weeks,all rats were weighed and fasting blood glucose（ FBG）,fasting insulin（ FINS）,glucagon（ GC） were tested. Insulin resistance index（ HOMA-IR） and insulin secretion index（ HOMA-β） were calculated. Immunofluorescence was used to analyze the distribution and localization of islet β / α cells in the pancreas. Results The FGB and weight of the Glibenclamide group and TCM group were significantly decreased after treatment,and decrease of FBG at the same time was more significant compared with the Model group（ P〈0. 05 or P〈0. 01）. Compared with the Model group,the GC levels of the Glibenclamide group and TCM group were significantly lower（ P〈0. 01）,while FINS,HOMA-β levels were significantly higher（ P〈0. 05 or P〈0. 01）. The FINS,HOMA-IR,HOMA-β levels of the Glibenclamide group were significantly higher than those of the TCM group（ P〈0. 05）. Immunofluorescence test showed that the number of islet β cells in the TCM group were significantly more while the number of islet α cells was reduced than that of the Model group and Glibenclamide group. Conclusion JPXKF could increase FINS,decrease GC,reduce insulin resistance and regulate the distribution and number of islet α,β cells in T2 DM rat,which may be one of the mechanisms.