补脾与醒脾药物对饮食诱导肥胖大鼠瘦素抵抗的调节作用

The regulation of strengthening the spleen and Xingpi drugs on the leptin resistance of dietinduced Obesity Rats

目的观察补脾、醒脾药物对饮食诱导肥胖(DIO)大鼠肥胖及瘦素抵抗的影响。方法 Wister大鼠120只,制作DIO大鼠40只和DIO-R大鼠10只,DIO大鼠分为DIO模型组、西布曲明组、醒脾组、补脾气组4组,分别以生理盐水、西布曲明、醒脾药物(藿香、佩兰、高良姜)、补脾气药物(白术、云苓、黄芪)灌胃,空白对照组与DIO-R组予生理盐水。灌胃16周后,测定体重,神经肽(NPY)、血清和脂肪匀浆中瘦素(leptin),脂肪匀浆中细胞因子转录负调节因子(SOCS-3)。结果 DIO模型组与DIO-R组比较,体重、NPY明显升高,血清和脂肪匀浆瘦素降低(P<0.05),SOCS-3升高(P<0.05,P<0.01)。药物干预后,各药物组体重有所下降。补脾气组治疗后体重、体重差值、NPY(P<0.01)、SOCS-3水平(P<0.05)减低,血清和脂肪匀浆瘦素水平升高(P<0.01),且在体重减低,血清瘦素升高方面明显优于西布曲明和醒脾组。结论 DIO大鼠存在瘦素抵抗,补脾药物能减轻肥胖,提高瘦素水平,抑制瘦素抵抗。

Objective To observe the effects of strengthening the spleen and Xingpi drugs on obesity and leptin resistance in DIO rats. Methods According to 120 Wister rats, we produced a rat model of 10 as a control group, given the basal feed, the remai- ning 110 given high fat and high nutrition diet for 17 weeks, according to body weight, obtained DIO rats 40 and DIO - R rats 10, DIO rats were divided into DIO model group, sibutramine group, Xingpi group, make up temper group, respectively, with saline, sibutramine, Xingpi drugs （rugosa, Perrin, galangal）make up temper drugs （Bai Zhu, Yun Ling, Astragalus） to gavage, given the control group and DIO -R group normal saline. Gavage for 16 weeks, the weight differences was measured in each group, the neuropeptide （NPY）and leptin in blood was measured, the leptin and SOCS -3 in fat was measured. Results （1） Compared DIO model group and DIO - R group, body weight was significantly higher （ P 〈 0.01 ）, NPY significantly increased （ P 〈 0.01 ）, leptin in the serum and adipose homogenate decreased （P 〈 0.05 ）, SOCS - 3 increased （ P 〈 0.05 ）. （2） After drug interven- tion, body weight of each drug group declined. Temper in the complement treatment group, the weight, the weight difference, NPY and SOCS- 3 levels （P 〈 0.05） decreased （P 〈 0.01 ）, leptin levels in serum and fat homogenates elevated （P 〈 0.01 ）, and it is superior to sibutramine and Xingpi group in body weight loss and increased serum leptin high . Conclusion （ 1 ） DIO rats have leptin resistance. （2） Spleen drugs can reduce obesity, increase leptin levels, inhibit leptin resistance, superior to Xingpi drugs.