采用大孔树脂富集内生真菌Hyalodendriella sp.中的Botrallin和TMC-264(英文)

Enrichment of Botrallin and TMC-264 from Endophytic Fungus Hyalodendriella sp. by Macroporous Resins

研究了6种大孔吸附树脂对内生真菌Hyalodendriella sp.Ponipodef12中活性成分Botrallin和TMC-264的富集作用,其中树脂SP825L表现出最好的吸附和解吸附作用。采用树脂SP825L,优化的吸附条件为:洗脱系统为水,洗脱体积为28 BV、流速为3 BV/h、p H值为5.0,温度为25℃;优化的解吸附条件为:洗脱系统为乙醇-水(50∶50,v/v)、洗脱体积为7 BV、流速为4 BV/h。从1600 mg乙酸乙酯粗提物中得到150 mg产物,Botrallin和TMC-264的含量由树脂处理前的2.18%和0.65%分别提高到处理后的20.66%和6.58%,Botrallin和TMC-264的回收率分别为88.85%和94.90%。结果表明,采用树脂SP825L从粗提物中富集Botrallin和TMC-264是一种有效的方法,有利于将来从内生真菌Hyalodendriella sp.Ponipodef12中大规模制备Botrallin和TMC-264。

The present study described a rapid and cost-effective method for the enrichment of botrallin and TMC-264 as major bioactive dibenzo-α-pyrones in the endophytic fungus Hyalodendriella sp. Ponipodef12 by macroporous resins. Resin SP825 L displayed the best adsorption and desorption properties for botrallin and TMC-264 among 6 resins. The optimal conditions for resin SP825 L adsorption of botrallin and TMC-264 were processing bed volumes（BV） as 28 BV,flow rate as 3 BV/h,p H value as 5. 0,and temperature as 25 ℃. Those for desorption were ethanol-water elution system at the ratio of 50 ∶ 50（v/v）,eluent volume as 7 BV,and flow rate as 4 BV/h. After one round treatment with resin SP825 L,the contents of botrallin and TMC-264 were increased from 2. 18% and 0. 65% in the crude extract to 20. 66%and 6. 58% in the final product,respectively. About 150 mg of the final product was obtained from 1600 mg of crude ethyl acetate extract. The recoveries for botrallin and TMC-264 were 88. 85% and 94. 90%,respectively. The results demonstrated that resin SP825 L chromatography can be an appropriate strategy for obtaining botrallin and TMC-264 from the crude extract. The optimized process will be beneficial for large-scale preparation of botrallin and TMC-264 from Hyalodendriella sp. Ponipodef12.