高速逆流色谱分离纯化雷公藤中的雷公藤红素

Preparation of Celastrol from Tripterygium wilfordii Hook. F. by High-speed Countercurrent Chromatography

以雷公藤植物粗提物为原料,建立了高速逆流色谱分离纯化雷公藤红素的分离纯化方法。优化了两相溶剂体系的组成及配比。优化后的分离纯化溶剂体系为正己烷-乙酸乙酯-甲醇-水,其体积之比为2∶3∶3∶2(上相为固定相,下相为流动相),实验温度为室温,主机转速为800 rpm,正向洗脱,流动相流速为2.0 m L/min。目标产物的分离时间较短、产品纯度高(97.5%)、分离过程稳定。

A single-step preparative high-speed countercurrent chromatography（HSCCC） method was developed for the separation and purification of celastrol from the extract of Tripterygium wilfordii Hook. F.（TWHF）. The results showed that under the selected solvent system（n-hexane/ethyl acetate/methanol/water = 2∶ 3∶ 3∶ 2,V/V/V/V,heavy phase as mobile phase and light phase as stationary phase）,flow rate of the mobile phase（2. 0 m L/min） and clockwise rotation,the mentioned compound was effectively separated at 25. 0 ℃ in rotational speed of 800 rpm. The developed HSCCC method was quick,stable and efficient.