松茸多糖TMSP-5亚组分Ⅱ的美白活性及途径研究

Study on the Whitening Activity and Pathway of Tricholoma matsutake TMSP-5 Ⅱ

本文初步研究了松茸多糖TMSP-5亚组分Ⅱ的美白作用机理。采用酪氨酸酶抑制实验、细胞水平黑色素测定实验来综合评价TMSP-5亚组分Ⅱ的美白功效;选定MITF、POMC和TYR三个靶基因作为研究对象,通过实时定量PCR实验,进一步阐明TMSP-5亚组分Ⅱ的美白作用靶点。结果显示,TMSP-5亚组分Ⅱ对酪氨酸酶具有一定的抑制作用,125μg/m L时酪氨酸酶抑制率为56.5%;不同浓度(3、30、300μg/m L)的TMSP-5亚组分Ⅱ样品能成剂量依赖的方式抑制B16细胞内黑色素水平,当浓度为300μg/m L时,TMSP-5亚组分Ⅱ样品能够减少黑色素的含量,且优于熊果苷;TMSP-5亚组分Ⅱ对MITF、POMC、TYR的表达水平均起下调作用,从而影响了黑素的形成。结果表明,TMSP-5亚组分Ⅱ样品具有一定的美白功效。TMSP-5亚组分Ⅱ样品对酪氨酸酶生成的重要基因和主要影响因子的基因水平的表达起到抑制作用,从而减少酪氨酸酶的量。

The mechanism of whitening activity of Tricholoma matsutake TMSP-5II was studied in this paper. The evaluation of whitening activity was performed by Tyrosinase Inhibitory Activity（TIA） and melanin determination. The results showed that TMSP-5II can inhibit tyrosinase activity,and the inhibition rate was 56% with the concentration of 125 μg /m L. In addition,TMSP-5II can significantly inhibit the melanin production of B16 in a dose dependent way,and the inhibitory activity was better than that of arbutin. MITF,POMC and TYR were selected to perform the real-time quantitative PCR（RT-PCR） experiments in order to determine the possible targeting gene of TMP-5II and the results indicated that TMSP-5II played an important role in the reduction of expression levels of MITF,POMC and TYR,leading to a decreased melanin production. Therefore,TMSP-5II reduced the gene expression of the proteins that play an essential role in the production of melanin.