摘要
目的:研究山药多糖对人肝癌HepG2细胞葡萄糖消耗能力和胰岛素抵抗的影响。方法:采用胰岛素(1×10-7mol/L)持续作用于HepG2细胞24 h以复制细胞胰岛素抵抗模型。将正常HepG2细胞分为正常对照(常规培养液)组、二甲双胍(0.01 mg/ml)组与山药多糖高、中、低浓度(质量浓度分别为1.00、0.10、0.01 mg/ml)组;将胰岛素抵抗HepG2细胞分为模型(常规培养液)组、二甲双胍(0.01 mg/ml)组与山药多糖高、中、低浓度(质量浓度分别为1.00、0.10、0.01 mg/ml)组,另设正常对照(正常细胞,常规培养液)组。加入相应药物后作用24 h,倒置显微镜下观察正常细胞与模型细胞的形态;测定细胞葡萄糖消耗量(ΔGC),MTT法测定单位细胞ΔGC(ΔGC/OD)。结果:与正常对照组比较,山药多糖高、中、低浓度组正常细胞ΔGC增加,ΔGC/OD升高,差异有统计学意义(P<0.01);模型组细胞ΔGC减少,ΔGC/OD降低,差异有统计学意义(P<0.01)。与模型组比较,山药多糖高、中、低浓度组细胞ΔGC增加,ΔGC/OD升高,差异有统计学意义(P<0.01)。正常Hep G2细胞与胰岛素抵抗Hep G2细胞的形态未见明显差异。结论:山药多糖能改善HepG2细胞的葡萄糖消耗能力,并且可以增强细胞对胰岛素的敏感性,具有体外降糖作用。
OBJECTIVE: To study the effects of Dioscorea japonica polysaccharide on glucose consumption capacity and insu- lin resistance of human lung cancer HepG2 cells. METHODS: HepG2 cells were treated with insulin (1 × 10^-7 mol/L) continuously to induce insulin resistance model cells. Normal HepG2 ceils were divided into normal control group (routine culture), metformin group (0.01 mg/ml) and D. japonica polysaccharide high-dose, medium-dose and low-dose groups (1.00, 0.10, 0.01 mg/ml) ; model HepG2 cells were divided into model group (routine culture), metformin group (0.01 mg/ml) and D. japonica polysaccha- ride high-dose, medium-dose and low-dose groups (1.00, 0.10, 0.01 mg/ml), setting normal control group(normal cells, routine culture). They were treated with relevant medicine for 24 h, cytomorphology photos were observed by microscope; glucose consumption of cells (AGC)was determined, and glucose consumption of cells of unit cell (AGC/OD) by MTT assay. Morphology of normal cells and model cells were observed. RESULTS: Compared with normal control group, AGC and AGC/OD of normal cells were increased in D. japonica polysaccharide high-dose, medium-dose and low-dose groups; there was statistical significance (P〈 0.01). Compared with normal control group, AGC and AGC/OD were decreased in model group; there was statistical significance (P〈0.01). Compared with model group, those of D. japonica polysaccharide high-dose, medium-dose and low-dose groups were increased; there was statistical significance (P〈0.01). No obvious change of cytomorphology in normal HepG2 cells and insulin-re- sistant HepG2 cells was found. CONCLUSIONS: D. japonica polysaccharide can improve the ability of HepG2 cells to consume glucose, and can enhance the sensitivity of cells to insulin. D. japonica polysaccharide has a hypoglycemic effect in vitro.
出处
《中国药房》
CAS
北大核心
2015年第4期458-460,共3页
China Pharmacy
基金
国家自然科学基金资助项目(No.81274101)
关键词
糖尿病
山药多糖
HEPG2细胞
胰岛素抵抗
降糖活性
Diabetes
Dioscorea japonica polysaccharide
HepG2 cells
Insulin resistance
Hypoglycemic activity