山药多糖对人肝癌HepG2细胞葡萄糖消耗能力及胰岛素抵抗的影响

Effects of Dioscorea japonica Polysaccharide on Glucose Consumption Capacity and Insulin Resistance of Human Lung Cancer HepG2 Cells

目的:研究山药多糖对人肝癌HepG2细胞葡萄糖消耗能力和胰岛素抵抗的影响。方法:采用胰岛素(1×10-7mol/L)持续作用于HepG2细胞24 h以复制细胞胰岛素抵抗模型。将正常HepG2细胞分为正常对照(常规培养液)组、二甲双胍(0.01 mg/ml)组与山药多糖高、中、低浓度(质量浓度分别为1.00、0.10、0.01 mg/ml)组;将胰岛素抵抗HepG2细胞分为模型(常规培养液)组、二甲双胍(0.01 mg/ml)组与山药多糖高、中、低浓度(质量浓度分别为1.00、0.10、0.01 mg/ml)组,另设正常对照(正常细胞,常规培养液)组。加入相应药物后作用24 h,倒置显微镜下观察正常细胞与模型细胞的形态;测定细胞葡萄糖消耗量(ΔGC),MTT法测定单位细胞ΔGC(ΔGC/OD)。结果:与正常对照组比较,山药多糖高、中、低浓度组正常细胞ΔGC增加,ΔGC/OD升高,差异有统计学意义(P<0.01);模型组细胞ΔGC减少,ΔGC/OD降低,差异有统计学意义(P<0.01)。与模型组比较,山药多糖高、中、低浓度组细胞ΔGC增加,ΔGC/OD升高,差异有统计学意义(P<0.01)。正常Hep G2细胞与胰岛素抵抗Hep G2细胞的形态未见明显差异。结论:山药多糖能改善HepG2细胞的葡萄糖消耗能力,并且可以增强细胞对胰岛素的敏感性,具有体外降糖作用。

OBJECTIVE： To study the effects of Dioscorea japonica polysaccharide on glucose consumption capacity and insu- lin resistance of human lung cancer HepG2 cells. METHODS： HepG2 cells were treated with insulin （1 × 10^-7 mol/L） continuously to induce insulin resistance model cells. Normal HepG2 ceils were divided into normal control group （routine culture）, metformin group （0.01 mg/ml） and D. japonica polysaccharide high-dose, medium-dose and low-dose groups （1.00, 0.10, 0.01 mg/ml） ; model HepG2 cells were divided into model group （routine culture）, metformin group （0.01 mg/ml） and D. japonica polysaccha- ride high-dose, medium-dose and low-dose groups （1.00, 0.10, 0.01 mg/ml）, setting normal control group（normal cells, routine culture）. They were treated with relevant medicine for 24 h, cytomorphology photos were observed by microscope; glucose consumption of cells （AGC）was determined, and glucose consumption of cells of unit cell （AGC/OD） by MTT assay. Morphology of normal cells and model cells were observed. RESULTS： Compared with normal control group, AGC and AGC/OD of normal cells were increased in D. japonica polysaccharide high-dose, medium-dose and low-dose groups; there was statistical significance （P〈 0.01）. Compared with normal control group, AGC and AGC/OD were decreased in model group; there was statistical significance （P〈0.01）. Compared with model group, those of D. japonica polysaccharide high-dose, medium-dose and low-dose groups were increased; there was statistical significance （P〈0.01）. No obvious change of cytomorphology in normal HepG2 cells and insulin-re- sistant HepG2 cells was found. CONCLUSIONS： D. japonica polysaccharide can improve the ability of HepG2 cells to consume glucose, and can enhance the sensitivity of cells to insulin. D. japonica polysaccharide has a hypoglycemic effect in vitro.