羟苯磺酸钙对急性心肌梗死模型大鼠心肌的保护作用

Cardioprotective Effects of Calcium Dobesilate on Acute Myocardial Infarction Model Rats

目的:研究羟苯磺酸钙对急性心肌梗死模型大鼠心肌的保护作用。方法:通过结扎冠状动脉前降支术建立急性心肌梗死大鼠模型。将模型大鼠随机分为模型组(生理氯化钠溶液),羟苯磺酸钙低、中、高剂量(200、400、800 mg/kg)组,阳性对照(链激酶4×104U/kg)组,每组10只,在梗死发生后立即尾静脉注射相应药物。采用化学发光法检测各组大鼠术前和给药后4、24、48、72h血浆中肌酸激酶、肌钙蛋白、血管内皮素的浓度,采用氯化硝基四氮唑磷酸缓冲液染色观察并计算给药后72 h小鼠心肌梗死范围。结果:与术前比较,模型组大鼠各时间点血浆中肌酸激酶、肌钙蛋白、血管内皮素浓度均明显增加,差异具有统计学意义(P<0.05)。与模型组比较,羟苯磺酸钙低、中、高剂量组和阳性对照组大鼠各时间点血浆中肌酸激酶、肌钙蛋白、血管内皮素浓度均减少,且与给药剂量和时间呈正相关;羟苯磺酸钙中、高剂量组和阳性对照组大鼠心肌梗死范围均明显减小,差异具有统计学意义(P<0.01),羟苯磺酸钙低剂量组大鼠心肌梗死范围差异无统计学意义(P>0.05)。结论:羟苯磺酸钙对急性心肌梗死模型大鼠的心肌具有保护作用,可能与抑制血管内皮素过量释放有关。

OBJECTIVE： To study the cardioprotective effects of calcium dobesilate on acute myocardial infarction mode rats. METHODS ： Acute myocardial infarction model was induced by ligation of anterior descending coronary, and then randomly divid- ed into model group （physiological solution of sodium chloride）, calcium dobesilate low-dose, medium-dose and high-dose groups （200, 400, 800 mg/kg） and positive control group （streptokinase 4× 10^4 U/kg） with 10 rats in each group. After modeling, they were given relevant medicines intravenously via tail vein. The plasma concentrations of creatine kinase, troponin and endothelin were determined by chemiluminescence method before operation and 4, 24, 48 and 72 h after medication. The range of myocardial infarction 72 h after medication were observed and measured by nitro blue tetrazolium chloride phosphoric acid buffer solution. RE- SULTS ： Compared with before operation, the plasma concentrations of creatine kinase, troponin and endothelin were increased sig- nificantly in model group; there was statistical significance （P〈0.05）. Compared with model group, those of calcium dobesilate low-dose, medium-dose and high-dose groups and positive control group were decreased, which was positively correlated with drug dosage and time; the range of myocardial infarction were decreased significantly in calcium dobesilate medium-dose and high-dose groups and positive control group; there was statistical significance （P〈0.01）. The range of myocardial infarction had no statistical significance in calcium dobesilate low-dose group （P〉0.05）. CONCLUSIONS： Calcium dobesilate can protect the myocardium of acute myocardial infarction mode rats, which is associated with inhibiting excessive release of endothelin.