蛇葡萄素钠协同卡铂对人肺腺癌GLC-82细胞增殖的抑制作用

Inhibitory Effects of Ampelopsin Sodium Combined with Carboplatin on the Proliferation of Human Lung Adenocarcinoma Cell Line GLC-82

目的：研究蛇葡萄素钠（AMP-Na）协同卡铂对人肺腺癌GLC-82细胞增殖的抑制作用。方法：以25、50、100μg/ml AMP-Na,3.12、6.25、12.5、25、50μg/ml卡铂,25、50、100μg/ml AMP-Na＋3.12、6.25、12.5、25、50μg/ml卡铂作用于GLC-82细胞48h,采用MTT法测定细胞增殖抑制率。以25μg/ml卡铂,12.5、25、50、100μg/ml AMP-Na,12.5、25、50、100μg/ml AMP-Na＋25μg/ml卡铂作用干细胞48 h,采用流式细胞仪检测细胞凋亡率。结果：25~100μg/ml AMP-Na对GLC-82细胞的增殖具有抑制作用,且呈浓度依赖性。25μg/ml卡铂与AMP-Na（12.5、25、50、100μg/ml）对GLC-82细胞增殖的抑制作用较单用卡铂增强。结论：AMP-Na单用对GLC-82细胞的增殖具有一定的抑制作用;与卡铂合用,对GLC-82细胞增殖具有协同抑制效应。

OBJECTIVE： To study the inhibitory effects of ampelopsin sodium （AMP-Na） combined with carboplatin on the proliferation of human lung adenocarcionma cell line GLC-82. METHODS： After treated with 25, 50 and 100 μg/ml AMP-Na, 3.12, 6.25, 12.5, 25 and 50 μg/ml carboplatin, 25, 50, 100 pg/ml AMP-Na＋3.12, 6.25, 12.5, 25 and 50 μg/ml carboplatin for 48 h, the inhibitory rate of GLC-82 cell proliferation was determined by MTT assay. After treated with 25 μg/ml carboplatin, 12.5, 25, 50, 100 μg/ml AMP-Na, 12.5, 25, 50, 100 μg/ml AMP-Na＋25 μg/ml carboplatin for 48 h, flow cytometry （FCM） was adop- ted to detect cell apoptotic rate. RESULTS： 25-100 μg/ml AMP-Na could inhibit the proliferation of GLC-82 cells in concentra- tion-dependent manner. The inhibitory effect of AMP-Na （12.5, 25, 50, 100 ~tg/ml） combined with carboplatin （25 p.g/ml） was stronger than that of carboplatin alone on the proliferation of GLC-82 cells. CONCLUSIONS ： AMP-Na alone can inhibit the prolif- eration of GLC-82 cells to certain extent, it also streghened inhibition of cell prolifertion when conbined with carboplatin.