摘要
[目的]该研究旨在探索番茄NAC转录因子与抗低温的关系。[方法]以番茄幼苗为试材,采用RT-PCR方法克隆NAC1基因N端保守域,并利用RT-q PCR技术检测该基因在4℃低温l处理下的表达情况。[结果]在番茄根、茎、叶中NAC1基因均能表达,表达量随低温处理时间的变化而发生相应的变化。在番茄的不同器官中NAC1表达水平也有所不同,根中的表达水平整体高于茎和叶片中。[结论]该研究为进一步研究番茄NAC1基因的生物学功能奠定理论基础。
[Objective] This study aimed to explore the relationship between NAC transcription factor and resistance to low temperature in tomato. [Method] Tomato seedlings were used as experimental materials to clone the N-terminal conserved domain of NAC1 gene with RT-PCR and investigate the expression patterns of NACl gene under 4 ℃ with real-time quantitative PCR （RT-qPCR）. [Result] NACl gene was expressed in roots, stems and leaves of tomato, but the expression level varied with the change of treatment duration. Moreover, the expression levels of NACl varied among different organs of tomato. The expression level of NACl in roots was much higher than that in stems and leaves. [Conclusion] This study laid a theoretical foundation for further investigation of the biological function of NAC1 gene in tomato.
基金
Supported by Beijing Fruit Vegetable Innovation Team of Modern Agriculture Industry Technology System(GCTDZJ2014033055)
National Natural Science Foundation of China(31171952)
Great Wall Scholar Training Program of Beijing Municipality(CIT&TCD20130323)~~
关键词
番茄
NAC
低温
Tomatoes
NAC
Low temperature
