杜氏盐藻促有丝分裂活化蛋白激酶DsMAPK的原核表达及纯化（英文）

Prokaryotic Expression and Purification of Ds MAPK from Dunaliella salina

盐藻是研究植物耐盐机制的重要模式生物,而MAPK基因在植物耐盐分子途径中起重要作用。该试验通过PCR扩增Ds MAPK基因的开放阅读框(ORF),并将其克隆至带有GST标签的原核表达载体p GS-21a,得到重组表达载体p GS-21a-MAPK。将重组表达载体转化E.coli BL21(DE3),IPTG诱导表达,然后对融合蛋白进行表达形式分析,利用GSTSefinoseTMKit进行纯化,所得产物进行SDSPAGE和Western blot鉴定。结果表明,该试验成功构建了重组表达载体p GS-21a-MAPK,经IPTG诱导后表达的融合蛋白与预期相符,纯化后的上清蛋白经Western blot检测显示该融合蛋白能与抗GST单克隆抗体特异性结合,具有良好的免疫学活性,Ds MAPK的成功表达为进一步在蛋白质水平上研究其在盐藻耐盐机制中的作用奠定了基础。

Dunaliella salina is an important model organism for investigating the salt tolerance mechanism of plant. MAPK is the key gene in the molecular pathway of salt tolerance of plant. In this experiment, the open reading frame （ORF） of DsMAPK gene was amplified by PCR. The target fragment was cloned in pGS-21a, a prokaryotic expression vector with GST-tag. The recombinant plasmid pGS-21a- DsMAPK was then transformed into E. coil BL21 （DE3）. The expression was induced with IPTG. Then the expression form of the recombinant protein was analyzed. The expression products were purified with GST-SefinoseTM Kit and identified with SDS-PAGE and Western blot. The results showed the recombinant expression vector pGS-21a-DsMAPK was constructed successfully, and the molecular weight of the expressed recombinant protein was as same as expected. Western blot analysis showed the purified recombinant protein could be identified specially by the anti- GST antibody and had a good immunological activity. The successful expression of DsMAPK will lay a basis for the further research on the role of DsMAPK in the salt tolerance mechanism at the protein level.