Study on Pathogenicity Difference of Plasmodiophora brassicae Under Different Temperature and pH Conditions

十字花科根肿病菌在不同温度、pH值条件下致病性差异研究（英文）

[Objective] This study was conducted to investigate the pathogenicity of Plasmodiophora brassicae on cabbage grown under different temperature and soil pH conditions. [Method] The pathogenicity of P. brassicae were tested at seven different temperatures and at six different soil pH values with the resting spore concentration of lx108 （spores/g） in the soil. The plant survival rate and incidence rate of clubroot were investigated after 90 d. [Result] The incidence rate of clubroot on cabbage among the different temperature sets varied in a descending order as follows： 30 ℃〉25 ℃〉20 ℃〉35 ℃〉15 ℃〉10 ℃〉5 ℃ at soil pH value of 6, indicating that the pathogenicity of P. brassicae was weak at 5 and 10 ~（3. The incidence rate increased with soil temperature increasing from 15 to 30 ℃, but decreased at 35 ℃. The incidence rates of clubroot were 80.36%, 100%, 65%, 10.77%, 3.23% and 0% at soil pH 4, 5, 6, 7, 8 and 9 at 25 ℃, respectively. The growth of cabbage was inhibited and the survival rate was reduced at pH 4.The incidence rates of clubroot were low at pH value of 7 and 8, and was 0% at pH 9. The Chinese cabbage grew better at pH value of 5 and 6, but had high incidence rates of clubroot. [Conclusion] The results revealed that the incidence rate of clubroot on cabbage was closely related to the temperature and soil pH.

[目的]探讨十字花科白菜根肿病菌在不同温度、pH值条件下致病性。[方法]采用根肿病菌和土壤混合法,在休眠孢子浓度1×108（个/g土）条件下,设置7个温度及6个土壤pH值梯度,进行根肿病菌对白菜致病性测定,90 d后拔根调查根肿病菌侵染发病情况。[结果]在pH值6时,不同温度梯度间白菜根肿病发病株率存在较大差异,其发病株率从高到低的温度为30℃〉25℃〉20℃〉35℃〉15℃〉10℃〉5℃。5℃和10℃条件下,其根肿病菌侵染力弱,不发病或发病株率较低;15~30℃条件下随温度升高发病率增加,但在35℃条件下,发病株率降低。温度25℃条件下,pH值为4、5、6、7、8、9的白菜发病株率分别为80.36%、100%、65%、10.77%、3.23%、0.0%。pH值4发病率虽高,但白菜生长受抑制,生长弱,存活率低。pH值7和8时,发病率较低,pH值9时没有发病,在pH值5和6时,植株生长良好,发病率也较高。[结论]该研究结果表明根肿病菌侵染发病与温度、土壤pH值关系密切。