ARTP诱变选育葡萄糖氧化酶高产菌株及发酵条件优化

ARTP mutation breeding of glucose oxidase-producing strains and optimization of fermentation conditions

利用常压室温等离子体诱变技术对产葡萄糖氧化酶的黑曲霉菌株进行诱变处理,通过平板筛选以及摇瓶复筛选出8株酶活较高的菌株,其中产酶活力最高的突变株为PCTC-8,酶活达到14.36 U/m L,较初始菌株的酶活提高了117.25%。然后在优化培养基的基础之上通过单因素实验对诱变菌株的发酵条件进行优化,最终确定最优的发酵条件为:接种量10%,装液量30 m L,种龄24 h,发酵时间48 h,转速225 r/min,在此条件下最高酶活可达到93.26 U/m L。

An atmospheric and room temperature plasma （ARTP） jet was used to mutagenize glucose oxidase-producing strains from Aspergillus niger. Eight strains which had higher glucose oxidase production capacity were selected through plate screening and flask fermentation experiments. The glucose oxidase activity of the best producer, strain PCTC-8, reached 14.36 U/mL, which was higher 117.25% than that of original strain. Effects of the inoculum size, seed age, rotation speed, fermentation time and cultural medium volume on the glucose oxidase activity of the mutant strain was investigated carefully by signal factor experiment. The optimum fermentation conditions of PCTC-8 to produce glucose oxidase were as follows： inoculum size 10% , seed age 24 h, rotation speed 225 r/min, fermentation time 48 h and cultural medium volume 30 mL, respectively. Under these conditions the glucose oxidase activity of the best producer was as high as 93.26 U/mL.