一株孕酮转化工程菌的构建

Construction of genetically engineered strain for bioconversion of progesterone

孕酮既是临床常用药物、又是可的松等药物的重要中间体,因此简便而快速的生产孕酮方法对于甾体激素的生产具有重要意义。本文利用红平红球菌的胆固醇氧化酶基因,构建了重组质粒BL21/p ET28b-cho E、并在大肠杆菌中获得重组表达。该菌株能够以孕烯醇酮为底物、转化生产孕酮。经条件优化,对浓度为0.2 g/L的孕烯醇酮的转化率大于80%。

Progesterone is not only a commonly used drug in clinic, but also a kind of important medicine intermediate in cortisol or cortisone synthesis. Thus a convenient method to produce progesterone is important in steroid drug production. In this study, a recombinant plasmid BL21/pET28b-choE was constructed by cloning choE gene from Rhodococcus erythropolis CGMCC 1. 2352 to express cholesterol oxidase in E. coli. Results showed that this recombinant strain could transform pregnenolone to progesterone. When the concentration of pregnenolone was up to 0.2 g/L, the bioconversion efficiency was more than 80%.