生物素光亲和分子的设计合成及其对2'-O-炔丙基鸟苷的标记

Design and synthesis of photoaffinity biotin labelled 2'-O-propargyl-guanosine

本文基于光亲和生物素探针分子在小分子配体靶标确证领域的应用,设计并合成了带有叠氮基的光亲和生物素探针分子1,证实其能够通过点击化学反应,对N2-乙酰基-2'-O-炔丙基鸟苷9进行标记。为进一步对环核苷酸信使分子及反义寡聚核苷酸和si RNA等进行光亲和生物素标记提供了方法。

Photoaffinity labeling is widely applied to demonstrate targets of small molecule ligands. In this paper, biotin photoaffinity labeled molecule with propargyl group 1 has been designed and synthesized, followed it＇s labeling of N2-acetyl-2＇-O-propargyl guanosine 9 by ＂click chemistry＂. This technology presents delight development potential in labeling of second messenger cyclic nucleotide, antisense oligonucleotide or siRNA.