石韦总黄酮的大孔树脂纯化工艺优选

Optimization of Purification Technology for Total Flavonoids from Pyrrosiae Folium by Macroporous Resin

目的：优选石韦总黄酮的大孔树脂纯化工艺条件,为该成分的制剂开发提供参考。方法：采用紫外-可见分光光度法测定总黄酮含量,检测波长510 nm。以总黄酮含量为指标,通过静态吸附-洗脱试验筛选大孔树脂型号,利用单因素试验考察上样量、径高比、洗脱剂用量等因素对石韦总黄酮大孔树脂纯化工艺的影响。结果：D101型大孔树脂吸附-洗脱性能最好,最佳纯化工艺条件为树脂柱径高比1∶10-1∶8,树脂-生药（1∶2.73）,上样液质量浓度0.5 g·m L^-1,上样速度2 BV·h^-1,加水7 BV洗脱,加60%乙醇5 BV以流速4 BV·h^-1洗脱。石韦总黄酮纯度达59.06%。结论：D101型大孔树脂对石韦总黄酮具有良好的纯化效果,优选的纯化工艺合理、稳定可行。

Objective： To optimize purification process of total flavonoids from Pyrrosiae Folium by macroporous resin. Method： The content of total flavonoids was determined by UV-visible spectrophotometry, detection wavelength was 510 nm. With the content of total flavonoids as index, types of maeroporous resin were screened by static adsorption-elution test, single factor tests were adopted to optimize purification technology with sample volume, ratio of diameter to height, the amount of eluent and so on. Result： D101 macroporous resin showed better adsorptionlution effect than others, optimum purification technology was as following： heightdiameter ratio of 6-10, ratio of resin to crude drug 1： 2.73, the concentration of sample solution 0.5 g ·mL^-1, absorption velocity 2 BV ·h^-1, washed impurity with 8 BV of water, then eluted with 5 BV of 60% ethanol at 4 BV ·h^-1, collected eluent. Under these conditions, purity of total flavonoids was 59.06%. Conclusion： D101 maeroporous resin has good purification effects on total flavonoids from Pyrrosiae Folium, this optimized purification technology is reasonable, stable and feasible.