高良姜叶中的化学成分

Identification of Chemical Constituents Occurring in Leaves of Alpinia officinarum

目的:研究高良姜叶中的化学成分。方法:高良姜叶经低温烘干粉碎,粉末样品中加入甲醇(1∶50),超声提取3次,合并提取液。样品经适当稀释后,注入液相色谱-质谱联用仪(LC-MS/MS),在电喷雾离子源正离子模式下(ESI+),采用多反应监测模式(MRM)对可能的化合物进行针对性分析。结果:在高良姜叶的甲醇提取液中共鉴定出分属于两类成分的16个化合物,其中黄酮类物质白杨素、乔松素、杨芽黄素、芹菜素、高良姜素、高良姜素-3-O-甲醚、金合欢素、山柰酚、山柰素、槲皮素、异鼠李素、芦丁。二芳基庚烷类成分包括益智酮甲、益智醇、六氢姜黄素和hannokinol。结论:本研究利用液相色谱-质谱联用技术确定了高良姜叶中的16种化学成分,尚有多种二芳基庚烷类成分需要分离鉴定。

Objective： The current study aimed to identify the chemical constituents occurring in the leaves of Alpinia officinarum. Method： The collected leaves of A. officinarum were dried in an electric thermostatic drying oven at 40℃ overnight. The freshly dried leaves were smashed using a universal high-speed smashing machine and then sieved manually by an 80 mesh. The resulting fine powders and residue were mixed evenly. An aliquot （0.6 g） was weighed precisely and macerated with 30 mL of methanol and then ultrasonicated three times for 30 min each. For each uhrasonication extraction, the resulting extract solutions were centrifuged at 8 000 rpm for 10 min. One micro liter of supernatant was sampled and the remaining was discarded. The residue was extracted with methanol for 2 more times. The sampled methanol extracts （3 mL） were combined and centrifuged at 13 000 rpm for 10 min to obtain the supernatant fractions that were frozen at -40 ℃ until analysis. The extract solution was appropriately diluted with methanol before analysis. Finally, a 10 μL aliquot was injected into the LC-MS/MS system, which was operated in the positive ion ESI mode with selected reaction monitoring for all the potential analytes. Result： Sixteen chemicals, including flavonoids and diarylheptanoids, were identified using LC-MS/MS with selected reaction monitoring mode （MRM）. Twelve flavonoids were chrysin, pinocembrin, tectochrysin, apigenin, galangin, 3-methylgalangin, acacetin, kaempferol, kaempferide, quercetin, isorhamnetin and rutin, respectively. Four diarylheptanoids included yakuchinone A, oxyphyllacinol, hexahydrocurcumin and hannokinol. Conclusion： Sixteen chemicals were identified using LC-MS/MS and some unknown diarylheptanoids need to be identified in the future.