芎汤超临界提取物中藁本内酯、正丁基苯酞和洋川芎内酯A在大鼠体内排泄动力学

Excretion Kinetics of Ligustilide,Butylphthalide and Senkyunolide A in Supercritical CO_2 Extraction Part from Xiongqiong Tang in Rats

目的：考察大鼠灌服芎汤超临界提取物后,其有效成分藁本内酯、正丁基苯酞和洋川芎内酯A在体内的排泄动力学规律。方法：将超临界部位对SD大鼠灌胃给药后,按不同时间段收集大鼠的尿液和粪样,对以上3种成分进行HPLC测定,绘制其尿和粪样排泄规律曲线,考察排泄特征。结果：藁本内酯0-24 h尿排泄率逐渐增高,24 h后逐渐降低,在粪样中36-48 h排泄率最高,此后逐渐降低;正丁基苯酞在尿液中12-48 h排泄率较高;洋川芎内酯A在粪样中12-48 h排泄率较高。结论：尿液中藁本内酯和正丁基苯酞,粪样中藁本内酯和洋川芎内酯A累积排泄量-时间曲线、平均排泄率-时间曲线比较一致,反映出共性的排泄特征,可为芎汤超临界提取物的整体排泄特征提供参考。

Objective： To investigate the excretion kinetics of the effective components of ligustilide, butylphthalide and senkyunolide A in supercritieal CO2 extraction part after oral administration of Xiongqiong Tang in rats. Method： The urine and feces samples of rats were collected according to different time periods after oral administration, the concentration of the above three compounds were determined by HPLC, the excretion curves were established and the excretion characteristics were studied using 3P 97 pharmacokinetic software. Result： The excretion curves showed that the urinary excretion rate of ligustilide increased gradually from 0 to 24 h, and then decreased after that, while in the feces the excretion rate of ligustilide approached the maximum from 36 h to 48 h and decreased whereafter; the urinary excretion rate of butylphthalide approached to the maximum gradually from 12 h to 48 h; the excretion rate of senkyunolide A in feces approached the maximum from 12 h to 48 h. Conclusion： The cumulative excretion-time curves and the average excretion rate-time curves of ligustil＇ide are in accordance with butylphthalide in urine, so are the ligustilide and senkyunolide A in feces, which reflectes a kind of common excretion characteristics. These findings provide reference for investigating the integral excretion pattern of Xiongqiong Tang.