补中益气汤治疗脾气虚证大鼠肌无力的机制研究

Mechanism of Buzhong Yiqi Tang in Treatment of Spleen Qi Deficiency Syndrome Amyosthenia in Rats

目的：研究脾气虚证大鼠体重、肛温、耐寒冷存活百分率、游泳时间和骨骼肌磷酸化肌球蛋白轻链表达变化,明确补中益气汤治疗脾气虚证大鼠肌无力的可能机制。方法：清洁级Wistar大鼠,采用饮食不节和劳倦过度结合法建立脾气虚证模型,单日喂食甘蓝15-20 g/只,双日胃饲猪油脂3 m L/只,共15 d10造模成功后,实验分为正常组、脾气虚组和补中益气汤组（高、中、低3个剂量组,剂量分别为7.10,3.55,1.78 g·kg^-1）,灌胃给药10 d10分别检测大鼠体重、肛温、耐寒冷存活率、游泳时间,Western blot检测骨骼肌中磷酸化肌球蛋白轻链的表达,比较实验结束前后数值变化。结果：与正常组相比,脾气虚组大鼠的体重、肛温、耐寒冷存活率、游泳时间及磷酸化肌球蛋白轻链表达均显著下降（P〈0.05）;与脾气虚组相比,中、高剂量补中益气汤组的体重、肛温、耐寒冷存活率、游泳时间及磷酸化肌球蛋白轻链表达均显著上升（P〈0.05）。结论：补中益气汤使磷酸化肌球蛋白轻链均表达升高可能是其治疗脾气虚大鼠肌无力的一个重要机制。

Objective： To study the possible mechanism of Buzhong Yiqi Tang in treatment of spleen Qi deficiency syndrome amyosthenia in rats. Method： A combined method of combined techniques and overstrain were used to establish a spleen Qi deficiency model in rat. Cabbage 15-20 g was given to rats every day, 3 mL pig grease was orally given to rats in every other day for 15 days. After a successful modeling, the rats were divided into normal control group, spteen Qi deficiency group, Buzhong Yiqi Tang treatment group （high dose, middle dose, and low dose of 7.10, 3.55, 1.78 g .kg-1 respectively） , and Buzhong Yiqi Tang was orally administrated for 10 days. The weight, rectal temperature, resistance to cold survival percentage and swimming time of rat were detected, and the expression of p-MLC was detected by Western blot, the value was compared before and after the treatment. Result： The weight, rectal temperature, resistance to cold survival percentage, swimming time and the expression of p-MLC in model group had a significant decrease compared to normal control group. The above mentioned indexes in middle dose and high dose groups of Buzhong Yiqi Tang had a significant increase compared to model group. Conclusion： Buzhong Yiqi Tang showed an up-regulation expression of p-MLC, which may be an mechanism for the treatment of spleen Qi deficiency syndrome amyosthenia in rats.