摘要
目的探索新佐剂CpG ODN在重组乙肝疫苗中的免疫效应及吸附状态。方法 CpG ODN佐剂与重组乙肝疫苗混合免疫小鼠,收集小鼠血清检测。通过免疫小鼠后的体液和细胞免疫应答水平来评价双佐剂乙肝疫苗对重组乙肝疫苗的增强作用。CpG ODN佐剂与乙肝疫苗在不同温度条件下混合吸附,计算在不同吸附时间下CpG ODN佐剂在乙肝疫苗中的吸附率。结果CpG ODN佐剂可以提高重组乙肝疫苗在体液及细胞中的免疫效果。CpG ODN佐剂在室温条件下1 h即可在乙肝疫苗中达到吸附平衡,于2~8℃放置5 d的条件下其吸附率约在50%左右。结论 CpG ODN能够增加乙肝疫苗的免疫应答,其免疫效果可提高5倍以上。
To investigate the adsorption state and immunological effects of CpG ODN adjuvant in hepatitis B vaccine, Balb/c mice were vaccinated by HBV vaccine and CpG ODN adjuvant. Humoral immunity titers and cellular immunity were detected to evaluate the immunogenicity of vaccines adjuvanted with CpG ODN.Furthermore, CpG ODN adsorption rates in different conditions were calculated. Data showed that CpG ODN could significantly increase the cellular and humoral immune effect of HBV vaccine in mice. When CpG ODN（500 μg/ml） was mixed and stored with recombinant Hepatitis B vaccine for 5 days at 2-8 ℃, the adsorption rate was approximate to 50%. The experiment proves that CpG ODN could significantly enhance the immune response of HBV in mice.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2015年第1期53-57,共5页
Immunological Journal
基金
国家高技术研究发展计划(2012AA02A404)
