小黑杨PnsICE1基因的植物表达载体构建及遗传转化

Construction of Plant Expression Vector of PnsICE1 Gene in Populus simonii×P. nigra and Transformation

[目的]构建PnsICE1基因的植物表达载体,并进行拟南芥遗传转化,以期为进一步研究小黑杨PnsICE1基因功能提供材料。[方法]以pROKⅡ载体为骨架,利用In-Fusion连接方法构建由Ca MV35S调控的小黑杨PnsICE1基因植物表达载体,用农杆菌介导法对拟南芥进行遗传转化。[结果]通过PCR检测,结果证明PnsICE1基因已整合到拟南芥基因组中,获得3株转PnsICE1基因拟南芥。[结论]植物表达载体的构建及转基因植株的获得为研究小黑杨PnsICE1基因功能提供材料。

[Objective]The goal of this study was to structure the plant expression vector of PnsICE1 and genetic transformation in Arabidopsis thaliana,in order to provide the data of further study on PnsICE1. [Method] Based on the pROKⅡ vector,the plant expression vector of PnsICE1 was constructed by the in-fusion method of attachment,in the vector,PnsICE1 gene was driven by promoter Ca MV35 S. PnsICE1 gene was transformed into Arabidopsis thaliana via Agrobacterium-mediated method. [Result] PCR detection indicated that the PnsICE1 was successfully integrated into Arabidopsis thaliana genome. [Conclusion]Construction of plant expression vector and acquisition of the transgenic plant in this study might be useful on study of PnsICE1 gene function.