摘要
目的:探讨血管内皮祖细胞移植对脓毒症大鼠的保护作用。方法:盲肠结扎穿孔法构建脓毒症大鼠模型,尾静脉移植血管内皮祖细胞,观察假手术组、模型组及血管内皮祖细胞移植组大鼠的尾动脉平均动脉压、体温、呼吸频率及脉率等主要生命体征,HE染色法检测肺部损伤情况、PAS染色法检测肾脏损伤情况,并用荧光显微镜观察大鼠肺部及肾脏血管内皮祖细胞的聚集情况。结果:盲肠结扎大鼠的尾动脉平均动脉压下降,体温、脉率及呼吸频率上升,但血管内皮祖细胞移植大鼠的生命体征都得到改善;肺部HE染色及肾脏PAS染色显示血管内皮祖细胞移植组肺部炎症细胞浸润及肾小球基底膜损伤均较模型组减轻,追踪血管内皮祖细胞的定位发现,在肺部及肾脏都有血管内皮祖细胞的聚集。结论:血管内皮祖细胞移植可以迅速聚集在血管损伤部位,对脓毒症大鼠的器官功能受损具有保护作用。
Objective: To investigate the protecting effects of endothelial progenitor cells transplantation on sepsis in rats. Methods: Sepsis rat model was constructed by cecal ligation-peferation (CLP) and endothelial progenitor cells were trans- planted through rat tail vain. Rats with sham operation were used as control. Mean arterial pressure, pulse rate, respiratory rate, and anal temperature were monitored 24 hours after operation. The lung injury was detected by hematoxylin-eosin stai- ning, and kidney injury was detected by periodic acid-schiff (PAS) staining. Fluorescence microscopy was used to track the en- dothelial progenitor cells in vivo. Results: The rats with CLP had down-regulated mean arterial pressure and up-regulated pulse rate, respiratory rate and anal temperature, while in rats with CLP and endothelial progenitor cells transplantation, all of these vital signs were improved. HE staining of lung biopsy showed that there are less inflammatory cells gathering in the endothelial progenitor cells transplanted rats comparing with sepsis rats. PAS staining of kidney biopsy showed that the injury of basement membrane was lightened if the sepsis rats were transplanted endothelial progenitor cells. Further investigation of tracking of endothelial progenitor cells revealed that there were endothelial progenitor cells gathering in the lung and kidney. Conclusions: The transplanted endothelial progenitor cells could quickly shift to the injury organ such as lung and kidney, and protected these organs from further destruction during sepsis.
出处
《海南医学院学报》
CAS
2015年第3期303-305,309,共4页
Journal of Hainan Medical University
基金
深圳市科创委知识创新计划项目(JCYJ20130401104112197)~~