骨桥蛋白在胆管癌中的表达及其与抗药性的关系

Expression of osteopontin in bile duct carcinoma and its relationship with drug resistance

目的:探讨骨桥蛋白在胆管癌组织中的表达及其与抗药性的关系。方法:回顾性分析本院收治的32例胆管癌患者及10例胆管炎患的临床资料者,采用实时定量PCR技术与免疫组织化学法(SP)测定胆管炎患者正常胆管组织、胆管癌组织及胆管癌癌旁组织中的骨桥蛋白mRNA(OPN-mRNA)的表达水平,比较两种组织中及不同临床病理之间的骨桥蛋白mRNA的表达水平差异;应用靶向RNA干扰骨桥蛋白基因的技术构建骨桥蛋白低表达细胞模型,并检测5-氟尿嘧啶与顺铂对癌细胞增殖的抑制作用。结果:对正常胆管组织、胆管癌组织、胆管癌癌旁组织中的OPN-mRNA进行测定,3种胆管组织中OPN-mRNA表达比较及两两组间比较,差异均具有统计学意义(P<0.05);不同肿瘤分期、不同分化程度、是否具有淋巴结转移的胆管癌患者的OPN-mRNA表达水平比较,差异具有统计学意义(P<0.05);肿瘤分期越高、分化程度越低、具有淋巴结转移的患者OPN-mRNA表达水平越高。沉默的OPN基因的胆管癌细胞OPN-mRNA表达明显低于未经沉默处理的胆管癌细胞,差异具有统计学意义(P<0.05);5-氟尿嘧啶、顺铂抑制沉默的OPN基因的胆管癌细胞OPN-mRNA表达明显低于未经沉默处理的胆管癌细胞,差异具有统计学意义(P<0.05)。结论:OPN-mRNA在胆管癌组织细胞中的表达明显高于正常组织,且与抗药性有一定的关系。

Objective; To investigate the expression of osteopontin in bile duct carcinoma and its relationship with drug resistance. Methods： Data of 32 cases of bile duct cancer were and 10 cases of cholangitis patients with clinicaldata were retro- spectively analyzed. Real-time quantitative PCR technique and immunohistochemical method （SP） determination of cholangitis were applied to detect the expression level of OPN-mRNA in normal bile duct tissues, cholangiocarcinoma tissues and paracan- cereus tissues. Differences in expression levels between the two groups were compared. RNA interference targeting osteopontin gene technology was applied to construct the low expression ofosteopontin cell model, and to detect the inhibitory effect of 5- fluorouracilandcisplatin on the proliferation of cancer cells. Results： Expression level of OPN-mRNA were detected in normal bile duct tissues, bile duct carcinoma, carcinoma of bile ductcarcinoma tissues, and significant differences were found a- mong the three bile duct tissues （P〈0.05）. Significant differences in OPN-mRNA expression level in bile tissues with differ- ent stages of carcinoma, differentiation degree, occurrence of lymph node metastasis were observed showing advanced stages of carcinoma, higher differentiation degree, occurrence of lymph node metastasis were correlated with higher OPN-mRNA expression level. Expression level of OPN-mRNA in OPN gene with silencing treatment was significantly lower than that in OPN gene without silencing treatment （P〈0.05）. 5- fluorouracil, cisplatin inhibiting bile duct cancer cell OPN-mRNA gene silence of OPN gene expression was significantly lower than that without the silent treatment ofeholangioearcinoma cells and the difference was statistically significant（P〈0.05）. Conclusion.. Expression level of OPN-mRNA in bile duct carcinoma tissues were significantly higher than that of normal tissue which may have certain relationship with drug resistance.