持续低温胁迫下大花蕙兰不同组织器官耐寒响应

The Response of Different Organs of Cymbidium hybridumto Continuous Low Temperature Stress

采用自然降温处理,分别测定了大花蕙兰地上部分和地下部分中丙二醛(MDA)含量、膜透性(CMP)、可溶性蛋白质(SP)含量、可溶性糖(SS)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性和过氧化氢酶(CAT)活性等与耐寒性有关的生理指标,比较地上部分和地下部分耐寒差异性,研究低温胁迫对大花蕙兰组培苗生理特性的影响。结果表明:POD活性、可溶性蛋白质含量和可溶性糖含量在反映大花蕙兰组培苗耐寒性方面作用明显,可作为其耐寒性鉴定指标;地上部分和地下部分在耐寒性提高时表现出不一致性,后者比前者表现出更强的耐寒性能。

Using the way of natural cooling process,seven indexes correlating with cold resistance including malondialdehyde（MDA）content,cell membrane permeability（CMP）,soluble protein（SP）content,soluble sugar（SS）content,superoxide dismutase（SOD）activity,peroxidase（POD）activity and catalase（CAT）activity of aboveground and underground were monitored.The effects of low temperature stress on physiological characteristics of Cymbidium hybridumplantlets in vitro were determined and the capacity between aboveground and underground in improving coldresistance for of Cymbidium hybridum were compared.The results showed that POD activity,soluble protein content and soluble sugar content significantly influenced the cold resistance and could be the indexes to identify the cold resistance in Cymbidium hybridumplantlets in vitro.The cold-resistance improvement of aboveground showed inconsistencies between different indexes to underground.The latter showed stronger ability of cold-resistance than the former.