复方二氧化氯对平菇褐斑病病原菌的杀菌效果及机理

The Germicidal Efficacy and Mechanism of a Compound Chlorine Dioxide to Pathogen of Oyster Mushroom Brown Spot

以平菇为试材,将采集到的平菇褐斑病标本进行了细菌菌株的分离纯化,纯化后的细菌菌株经回接感染试验和细菌形态学鉴定确定致病病原菌。通过悬液定量杀菌试验研究不同配方复方二氧化氯食用菌杀菌剂对平菇褐斑病病原菌的杀菌效果,筛选出有效的复方二氧化氯食用菌杀菌剂配方。最后从细菌细胞微观形态和细胞膜渗透性变化2个方面分析了复方二氧化氯的杀菌机理。结果表明:5mg/L二氧化氯和2.3mg/L的烷基糖苷的复配后对平菇褐斑病病原菌杀灭对数值可以达到5,杀菌作用显著。复方二氧化氯中的杀菌增效剂烷基糖苷对二氧化氯杀菌起到协同增效作用,可以降低二氧化氯的用量,复方二氧化氯对病原菌细胞超微结构和内含物的破坏能力大于二氧化氯,杀菌效果更好。

Taking oyster mushroom as test material,the bacteria strains were isolated from oyster mushroom infected by brown spot and pathogenic bacteria were indentified by artificial infection test and morphological identification.The optimum ingredient design of compound chlorine dioxide was determined by studying the bactericidal effects of bactericide with different ingredients on pathogenic bacteria of brown spot through using suspension quantitative germicidal test.At last the germicidal mechanism of compound chlorine dioxide was analyzed by the variation of micro morphology of bacterial cell determined by TEM and permeability of cell membrane indicated by soluble protein.The results showed that the killing log value of pathogen of oyster mushroom brown spot exposed to optimum compound ingredient with 5mg/L chlorine dioxide disinfectant and 2.3mg/L alkyl-polyglucoside（APG）could attain 5.The use of sterilization synergist APG in compound chlorine dioxide could decre-ase the dosage of chlorine dioxide thus reduce the affection to fruitbody.The damaged capacity of compound chlorine dioxide to micromorphology and inclusion of bacteria cells was more than chlorine dioxide.