摘要
目的:探讨兔视网膜缺血再灌注中视网膜神经节细胞凋亡及bcl-2表达的变化及苯甲酸雌二醇对其的影响。方法:将实验兔随机分为正常假手术组、缺血组、雌激素治疗组。缺血组和治疗组分为再灌注后3、6、12、24、72 h 5个时间段。制作兔视网膜缺血再灌注损伤模型,通过用缺口末端标记法检测视网膜组织神经节细胞凋亡的变化;免疫组织化学过氧化酶法检测不同时段视网膜组织中的bcl-2的表达变化。结果:雌激素治疗组视网膜神经节细胞凋亡阳性细胞数在再灌注后12、24和72 h均低于缺血组(P〈0.05-P〈0.01)。治疗组bcl-2阳性细胞数在再灌注12、24和72 h均较缺血组显著增加(P〈0.01)。结论:苯甲酸雌二醇可以增加视网膜缺血再灌注损伤时抗凋亡基因bcl-2在视网膜的表达,进而抑制细胞凋亡来实现其对视网膜缺血再灌注损伤的治疗作用。
Objective: To explore the effects of estrogen on retinal ganglion cell apoptosis and Bcl-2 expression in retinal ischemiareperfusion injury of rabbit. Methods: The animals were randomly divided into the sham operation group,ischemia group and estrogen treatment group,the ischemia group and estrogen treatment group were subdivided into 3,6,12,24 and 72 h time-point after reperfusion. The retinal ischemia-reperfusion injury model of rabbit was established. The retinal ganglion cell apoptosis was detected using TUNEL method,the bcl-2 expressions in retinal tissue at different time-points were detected by immunohistochemistry SABC method. Results: The number of TUNEL-positive retinal ganglion cells in estrogen treatment group at 12,24 and 72 h after reperfusion were less than that in ischemia group( P〈0. 05 to P〈0. 01). Compared with the ischemia group,the number of bcl-2 positive cells in estrogen treatment group significantly increased at 12,24 and 72 h after reperfusion( P〈0. 01). Conclusions: Estradiol benzoate can increase the bcl-2 anti-apoptosis gene expression in retinal ischemia-reperfusion injury,and play a therapeutic effect on retinal ischemia reperfusion injury through inhibiting the cell apoptosis.
出处
《蚌埠医学院学报》
CAS
2015年第1期11-14,共4页
Journal of Bengbu Medical College
基金
河北省唐山市科技计划资助项目(12140209A-47)
关键词
视网膜
再灌注损伤
苯甲酸雌二醇
凋亡
兔
retina
ischemia-reperfusion injury
estradiol benzoate
apoptosis
rabbits