雌激素对兔视网膜缺血再灌注损伤中视网膜神经节细胞凋亡及bcl-2表达的影响

The effect of estrogen on retinal ganglion cell apoptosis and Bcl-2 expression in retinal ischemia-reperfusion injury of rabbit

目的：探讨兔视网膜缺血再灌注中视网膜神经节细胞凋亡及bcl-2表达的变化及苯甲酸雌二醇对其的影响。方法：将实验兔随机分为正常假手术组、缺血组、雌激素治疗组。缺血组和治疗组分为再灌注后3、6、12、24、72 h 5个时间段。制作兔视网膜缺血再灌注损伤模型,通过用缺口末端标记法检测视网膜组织神经节细胞凋亡的变化;免疫组织化学过氧化酶法检测不同时段视网膜组织中的bcl-2的表达变化。结果：雌激素治疗组视网膜神经节细胞凋亡阳性细胞数在再灌注后12、24和72 h均低于缺血组（P〈0.05-P〈0.01）。治疗组bcl-2阳性细胞数在再灌注12、24和72 h均较缺血组显著增加（P〈0.01）。结论：苯甲酸雌二醇可以增加视网膜缺血再灌注损伤时抗凋亡基因bcl-2在视网膜的表达,进而抑制细胞凋亡来实现其对视网膜缺血再灌注损伤的治疗作用。

Objective： To explore the effects of estrogen on retinal ganglion cell apoptosis and Bcl-2 expression in retinal ischemiareperfusion injury of rabbit. Methods： The animals were randomly divided into the sham operation group,ischemia group and estrogen treatment group,the ischemia group and estrogen treatment group were subdivided into 3,6,12,24 and 72 h time-point after reperfusion. The retinal ischemia-reperfusion injury model of rabbit was established. The retinal ganglion cell apoptosis was detected using TUNEL method,the bcl-2 expressions in retinal tissue at different time-points were detected by immunohistochemistry SABC method. Results： The number of TUNEL-positive retinal ganglion cells in estrogen treatment group at 12,24 and 72 h after reperfusion were less than that in ischemia group（ P〈0. 05 to P〈0. 01）. Compared with the ischemia group,the number of bcl-2 positive cells in estrogen treatment group significantly increased at 12,24 and 72 h after reperfusion（ P〈0. 01）. Conclusions： Estradiol benzoate can increase the bcl-2 anti-apoptosis gene expression in retinal ischemia-reperfusion injury,and play a therapeutic effect on retinal ischemia reperfusion injury through inhibiting the cell apoptosis.